TY - JOUR
T1 - Matrix-specified differentiation of human decidua parietalis placental stem cells
AU - Sridharan, Indumathi
AU - Kim, Taeyoung
AU - Strakova, Zuzana
AU - Wang, Rong
N1 - Funding Information:
This research was supported by NIH (R01 NS047719).
PY - 2013/8/2
Y1 - 2013/8/2
N2 - To create suitable biological scaffolds for tissue engineering and cell therapeutics, it is essential to understand the matrix-mediated specification of stem cell differentiation. To this end, we studied the effect of collagen type I on stem cell lineage specification. We altered the properties of collagen type I by incorporating carbon nanotubes (CNT). The collagen-CNT composite material was stiffer with thicker fibers and longer D-period. Human decidua parietalis stem cells (hdpPSC) were found to differentiate exclusively and rapidly towards neural cells on the collagen-CNT matrix. We attribute this accelerated neural differentiation to the enhanced structural and mechanical properties of collagen-CNT material. Strikingly, the collagen-CNT matrix, unlike collagen, imposes the neural fate by an alternate mechanism that may be independent of beta-1 integrin and beta-catenin. The study demonstrates the sensitivity of stem cells to subtle changes in the matrix and the utilization of a novel biocomposite material for efficient and directed differentiation of stem cells.
AB - To create suitable biological scaffolds for tissue engineering and cell therapeutics, it is essential to understand the matrix-mediated specification of stem cell differentiation. To this end, we studied the effect of collagen type I on stem cell lineage specification. We altered the properties of collagen type I by incorporating carbon nanotubes (CNT). The collagen-CNT composite material was stiffer with thicker fibers and longer D-period. Human decidua parietalis stem cells (hdpPSC) were found to differentiate exclusively and rapidly towards neural cells on the collagen-CNT matrix. We attribute this accelerated neural differentiation to the enhanced structural and mechanical properties of collagen-CNT material. Strikingly, the collagen-CNT matrix, unlike collagen, imposes the neural fate by an alternate mechanism that may be independent of beta-1 integrin and beta-catenin. The study demonstrates the sensitivity of stem cells to subtle changes in the matrix and the utilization of a novel biocomposite material for efficient and directed differentiation of stem cells.
KW - Carbon nanotubes
KW - Collagen
KW - Neural differentiation
KW - Stem cells
UR - http://www.scopus.com/inward/record.url?scp=84880931720&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2013.07.002
DO - 10.1016/j.bbrc.2013.07.002
M3 - Article
C2 - 23850689
AN - SCOPUS:84880931720
SN - 0006-291X
VL - 437
SP - 489
EP - 495
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -