TY - JOUR
T1 - Mass fragmentographic determination of pyrazinamide and its metabolites in serum and urine
AU - Roboz, John
AU - Suzuki, Robert
AU - Yü, Ts'ai Fan
N1 - Funding Information:
This work was supported in part by Grants AM-00162 and 5POl CA-15936 by the National Institutes of Health, U.S.A.
PY - 1978/1/11
Y1 - 1978/1/11
N2 - A combined gas chromatographic-mass spectrometric technique is described for the simultaneous determination of pyrazinamide and its two main metabolites, pyrazinoic acid and 5-hydroxypyrazinoic acid. Serum (200 μl) is deproteinized and evaporated to dryness; urine (20 μl) is evaporated. The crude residues are silylated and selected ions are monitored in the chemical-ionization mode with isobutane as both chromatographic carrier and reagent gas. The sensitivity is 10 ng/ml for pyrazinamide and pyrazinoic acid and 20 ng/ml for the 5-hydroxy metabolite in a single analysis. Nicotinic acid and nicotinamide are internal standards. Both unchanged drug and metabolites were identified and quantified in the serum and urine of human subjects.
AB - A combined gas chromatographic-mass spectrometric technique is described for the simultaneous determination of pyrazinamide and its two main metabolites, pyrazinoic acid and 5-hydroxypyrazinoic acid. Serum (200 μl) is deproteinized and evaporated to dryness; urine (20 μl) is evaporated. The crude residues are silylated and selected ions are monitored in the chemical-ionization mode with isobutane as both chromatographic carrier and reagent gas. The sensitivity is 10 ng/ml for pyrazinamide and pyrazinoic acid and 20 ng/ml for the 5-hydroxy metabolite in a single analysis. Nicotinic acid and nicotinamide are internal standards. Both unchanged drug and metabolites were identified and quantified in the serum and urine of human subjects.
UR - http://www.scopus.com/inward/record.url?scp=0017903571&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(00)85145-6
DO - 10.1016/S0021-9673(00)85145-6
M3 - Article
C2 - 621253
AN - SCOPUS:0017903571
SN - 0021-9673
VL - 147
SP - 337
EP - 347
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - C
ER -