Mapping the single-cell landscape of acral melanoma and analysis of the molecular regulatory network of the tumor microenvironments

Zan He; Zijuan Xin; Qiong Yang; Chen Wang; Meng Li; Wei Rao; Zhimin Du; Jia Bai; Zixuan Guo; Xiuyan Ruan; Zhaojun Zhang; Xiangdong Fang; Hua Zhao

doi:10.7554/ELIFE.78616

Mapping the single-cell landscape of acral melanoma and analysis of the molecular regulatory network of the tumor microenvironments

Zan He
, Zijuan Xin
, Qiong Yang
, Chen Wang
, Meng Li
, Wei Rao
, Zhimin Du
, Jia Bai
, Zixuan Guo
, Xiuyan Ruan
, Zhaojun Zhang
, Xiangdong Fang
, Hua Zhao

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Acral melanoma (AM) exhibits a high incidence in Asian patients with melanoma, and it is not well treated with immunotherapy. However, little attention has been paid to the characteristics of the immune microenvironment in AM. Therefore, in this study, we collected clinical samples from Chinese patients with AM and conducted single-cell RNA sequencing to analyze the heterogeneity of its tumour microenvironments (TMEs) and the molecular regulatory network. Our analysis revealed that genes, such as TWIST1, EREG, TNFRSF9, and CTGF could drive the deregulation of various TME components. The molecular interaction relationships between TME cells, such as MIF–CD44 and TNFSF9–TNFRSF9, might be an attractive target for developing novel immunotherapeutic agents.

Original language	English
Article number	e78616
Journal	eLife
Volume	11
DOIs	https://doi.org/10.7554/ELIFE.78616
State	Published - Jul 2022
Externally published	Yes

Keywords

Acral melanoma
single-cell RNA sequencing
tumor microenvironment

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10.7554/ELIFE.78616

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title = "Mapping the single-cell landscape of acral melanoma and analysis of the molecular regulatory network of the tumor microenvironments",

abstract = "Acral melanoma (AM) exhibits a high incidence in Asian patients with melanoma, and it is not well treated with immunotherapy. However, little attention has been paid to the characteristics of the immune microenvironment in AM. Therefore, in this study, we collected clinical samples from Chinese patients with AM and conducted single-cell RNA sequencing to analyze the heterogeneity of its tumour microenvironments (TMEs) and the molecular regulatory network. Our analysis revealed that genes, such as TWIST1, EREG, TNFRSF9, and CTGF could drive the deregulation of various TME components. The molecular interaction relationships between TME cells, such as MIF{\textendash}CD44 and TNFSF9{\textendash}TNFRSF9, might be an attractive target for developing novel immunotherapeutic agents.",

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author = "Zan He and Zijuan Xin and Qiong Yang and Chen Wang and Meng Li and Wei Rao and Zhimin Du and Jia Bai and Zixuan Guo and Xiuyan Ruan and Zhaojun Zhang and Xiangdong Fang and Hua Zhao",

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language = "English",

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TY - JOUR

T1 - Mapping the single-cell landscape of acral melanoma and analysis of the molecular regulatory network of the tumor microenvironments

AU - He, Zan

AU - Xin, Zijuan

AU - Yang, Qiong

AU - Wang, Chen

AU - Li, Meng

AU - Rao, Wei

AU - Du, Zhimin

AU - Bai, Jia

AU - Guo, Zixuan

AU - Ruan, Xiuyan

AU - Zhang, Zhaojun

AU - Fang, Xiangdong

AU - Zhao, Hua

PY - 2022/7

Y1 - 2022/7

N2 - Acral melanoma (AM) exhibits a high incidence in Asian patients with melanoma, and it is not well treated with immunotherapy. However, little attention has been paid to the characteristics of the immune microenvironment in AM. Therefore, in this study, we collected clinical samples from Chinese patients with AM and conducted single-cell RNA sequencing to analyze the heterogeneity of its tumour microenvironments (TMEs) and the molecular regulatory network. Our analysis revealed that genes, such as TWIST1, EREG, TNFRSF9, and CTGF could drive the deregulation of various TME components. The molecular interaction relationships between TME cells, such as MIF–CD44 and TNFSF9–TNFRSF9, might be an attractive target for developing novel immunotherapeutic agents.

AB - Acral melanoma (AM) exhibits a high incidence in Asian patients with melanoma, and it is not well treated with immunotherapy. However, little attention has been paid to the characteristics of the immune microenvironment in AM. Therefore, in this study, we collected clinical samples from Chinese patients with AM and conducted single-cell RNA sequencing to analyze the heterogeneity of its tumour microenvironments (TMEs) and the molecular regulatory network. Our analysis revealed that genes, such as TWIST1, EREG, TNFRSF9, and CTGF could drive the deregulation of various TME components. The molecular interaction relationships between TME cells, such as MIF–CD44 and TNFSF9–TNFRSF9, might be an attractive target for developing novel immunotherapeutic agents.

KW - Acral melanoma

KW - single-cell RNA sequencing

KW - tumor microenvironment

UR - https://www.scopus.com/pages/publications/85136823018

U2 - 10.7554/ELIFE.78616

DO - 10.7554/ELIFE.78616

M3 - Article

C2 - 35894206

AN - SCOPUS:85136823018

SN - 2050-084X

VL - 11

JO - eLife

JF - eLife

M1 - e78616

ER -

Mapping the single-cell landscape of acral melanoma and analysis of the molecular regulatory network of the tumor microenvironments

Abstract

Keywords

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