Mapping the alterations in glutamate with GluCEST MRI in a mouse model of dopamine deficiency

Puneet Bagga, Rachelle Crescenzi, Guruprasad Krishnamoorthy, Gaurav Verma, Ravi Prakash Reddy Nanga, Damodar Reddy, Joel Greenberg, John A. Detre, Hari Hariharan, Ravinder Reddy

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Glutamate chemical exchange saturation transfer (GluCEST) MRI was used to measure metabolic changes in mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) by mapping regional cerebral glutamate. The GluCEST contrast following MPTP treatment was correlated with 1H-MR spectroscopy, motor function, and immunohistochemical measures. The GluCEST contrast was found to be significantly higher in the striatum and motor cortex of mice treated with MPTP than in controls (p < 0.001), which was confirmed by localized 1H-MR spectroscopy. Elevated striatal GluCEST was positively associated with local astrogliosis measured by immunohistochemistry for glial fibrillary acidic protein. Additionally, a negative correlation was found between motor function, measured by the four-limb grip strength test, and GluCEST of the striatum (R = −0.705, p < 0.001) and the motor cortex (R = −0.617, p < 0.01), suggesting a role of elevated glutamate in the abnormal cerebral motor function regulation. The GluCEST contrast and glial fibrillary acidic protein immunostaining were unaltered in the thalamus indicating glutamate elevation was localized to the striatum and the motor cortex. These findings suggest that in addition to measuring spatial changes in glutamate, GluCEST may serve as an in vivo biomarker of metabolic and functional changes that may be applied to the assessment of a broad range of neuropathologies. (Figure presented.). Read the Editorial Highlight for this article on page 346.

Original languageEnglish
Pages (from-to)432-439
Number of pages8
JournalJournal of Neurochemistry
Issue number3
StatePublished - 1 Nov 2016
Externally publishedYes


  • CEST
  • GFAP
  • MRI
  • astrogliosis
  • brain
  • glutamate
  • tyrosine hydroxylase


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