Abstract
In previous communications the authors reported that the eight RNA segments of influenza A/PR/8/34 (HON1) virus could be distinguished from corresponding segments of influenza A/Hong Kong/8/68 (H3N2) virus by migration on polyacrylamide urea gels. Examination of the RNA patterns of the two parent viruses and recombinants derived from them in concert with serological identification of surface proteins and analysis of the other proteins on sodium dodecyl sulfate gradient gels permitted the identification of the genes coding for hemagglutinin, neuraminidase, and the P1, P2, and P3 proteins. In the report the authors have extended these observations using similar techniques to examine other recombinants and have identified the genes coding for the remaining virus specific proteins. We demonstrate that segment 5 of PR8 virus (counting the slowest moving RNA segment as 1) and segment 6 of Hong Kong virus code for the respective nucleoproteins, and that segment 7 of both viruses codes for the membrane protein and RNA segment 8 codes for the nonstructural protein. This completes the mapping of the influenza A virus genome.
| Original language | English |
|---|---|
| Pages (from-to) | 307-313 |
| Number of pages | 7 |
| Journal | Journal of Virology |
| Volume | 20 |
| Issue number | 1 |
| State | Published - 1976 |
| Externally published | Yes |