TY - JOUR
T1 - Mapping of the human genes encoding cyclin H (CCNH) and the CDK- activating kinase (CAK) assembly factor MAT1 (MNAT1) to chromosome bands 5q13.3-q14 and 14q23, respectively
AU - Eki, Toshihiko
AU - Okumura, Katsuzumi
AU - Abe, Makoto
AU - Kagotani, Kazuhiro
AU - Taguchi, Hiroshi
AU - Murakami, Yasufumi
AU - Pan, Zhen Qiang
AU - Hanaoka, Fumio
N1 - Funding Information:
We thank Dr. Y. Xiong (University of North Carolina) for human MNAT1 plasmid, Dr. D. Reinberg (University of Medicine and Dentistry of New Jersey) for human cyclin H plasmid, and T. Mogi (RI-KEN) for PCR screening. The cooled CCD camera digital imaging system at Center for Molecular Biology and Genetics, Mie University, was used in this work. This work was supported by grants to the Life Science Research Project of RIKEN, the Science and Technology Agency of Japan, in part by a grant from the Ministry of Education, Science, Sports, and Culture of Japan to T.E., K.O., and F.H., and by funds from the Life and Health Medical fund and New York Community Trust to Z.-Q.P.
PY - 1998/1/1
Y1 - 1998/1/1
N2 - Cyclin-dependent kinases (CDKs), which play a key role in cell cycle control, are activated by the CDK-activating kinase (CAK), which activates cyclin-bound CDKs by phosphorylation at the specific threonine residue. Mammalian CAK contains three components: CDK7, cyclin H, and an assembly factor called MAT1. The CDK7-cyclin H-MAT1 complex is tightly associated with a multiprotein complex TFIIH, which plays a dual role in transcription and DNA repair. Here, we have determined chromosomal localizations of the human genes encoding cyclin H (CCNH) and MAT1 (HGMW-approved symbol MNAT1) to chromosome bands 5q13.3-q14 and 14q23, respectively, by using fluorescence in situ hybridization, somatic cell hybrid analyses, and mapping to the human YAC contigs.
AB - Cyclin-dependent kinases (CDKs), which play a key role in cell cycle control, are activated by the CDK-activating kinase (CAK), which activates cyclin-bound CDKs by phosphorylation at the specific threonine residue. Mammalian CAK contains three components: CDK7, cyclin H, and an assembly factor called MAT1. The CDK7-cyclin H-MAT1 complex is tightly associated with a multiprotein complex TFIIH, which plays a dual role in transcription and DNA repair. Here, we have determined chromosomal localizations of the human genes encoding cyclin H (CCNH) and MAT1 (HGMW-approved symbol MNAT1) to chromosome bands 5q13.3-q14 and 14q23, respectively, by using fluorescence in situ hybridization, somatic cell hybrid analyses, and mapping to the human YAC contigs.
UR - https://www.scopus.com/pages/publications/0031914863
U2 - 10.1006/geno.1997.5053
DO - 10.1006/geno.1997.5053
M3 - Article
C2 - 9465303
AN - SCOPUS:0031914863
SN - 0888-7543
VL - 47
SP - 115
EP - 120
JO - Genomics
JF - Genomics
IS - 1
ER -