Mapping of DNA-binding proteins along the yeast genome by UV-induced DNA-protein crosslinking

D. A. Papatsenko; I. V. Priporova; S. V. Belikov; V. L. Karpov

doi:10.1016/0014-5793(96)00091-9

Mapping of DNA-binding proteins along the yeast genome by UV-induced DNA-protein crosslinking

D. A. Papatsenko
, I. V. Priporova
, S. V. Belikov
, V. L. Karpov

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

UV-induced crosslinking of DNA-binding proteins to DNA in intact nuclei of Saccharomyces cerevisiae and subsequent 'protein image' hybridization were applied to map non-histone proteins along single-copy genes of yeast. We detected two polypeptides that most probably correspond to core subunits of yeast RNA-polymerase II in the coding region of transketolase gene (TKL2). Several non-histone proteins were also detected which bind to the upstream region of TKL2 gene, and to the intergenic spacer between cahnodulin (CMD1) and β-mannosyl transferase (ALG1) genes.

Original language	English
Pages (from-to)	103-105
Number of pages	3
Journal	FEBS Letters
Volume	381
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(96)00091-9
State	Published - 26 Feb 1996
Externally published	Yes

Keywords

DNA-binding protein
DNA-protein crosslinking
Saccharomyces cerevisiae
UV-light

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10.1016/0014-5793(96)00091-9

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title = "Mapping of DNA-binding proteins along the yeast genome by UV-induced DNA-protein crosslinking",

abstract = "UV-induced crosslinking of DNA-binding proteins to DNA in intact nuclei of Saccharomyces cerevisiae and subsequent 'protein image' hybridization were applied to map non-histone proteins along single-copy genes of yeast. We detected two polypeptides that most probably correspond to core subunits of yeast RNA-polymerase II in the coding region of transketolase gene (TKL2). Several non-histone proteins were also detected which bind to the upstream region of TKL2 gene, and to the intergenic spacer between cahnodulin (CMD1) and β-mannosyl transferase (ALG1) genes.",

keywords = "DNA-binding protein, DNA-protein crosslinking, Saccharomyces cerevisiae, UV-light",

author = "Papatsenko, \{D. A.\} and Priporova, \{I. V.\} and Belikov, \{S. V.\} and Karpov, \{V. L.\}",

year = "1996",

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doi = "10.1016/0014-5793(96)00091-9",

language = "English",

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AU - Papatsenko, D. A.

AU - Priporova, I. V.

AU - Belikov, S. V.

AU - Karpov, V. L.

PY - 1996/2/26

Y1 - 1996/2/26

N2 - UV-induced crosslinking of DNA-binding proteins to DNA in intact nuclei of Saccharomyces cerevisiae and subsequent 'protein image' hybridization were applied to map non-histone proteins along single-copy genes of yeast. We detected two polypeptides that most probably correspond to core subunits of yeast RNA-polymerase II in the coding region of transketolase gene (TKL2). Several non-histone proteins were also detected which bind to the upstream region of TKL2 gene, and to the intergenic spacer between cahnodulin (CMD1) and β-mannosyl transferase (ALG1) genes.

AB - UV-induced crosslinking of DNA-binding proteins to DNA in intact nuclei of Saccharomyces cerevisiae and subsequent 'protein image' hybridization were applied to map non-histone proteins along single-copy genes of yeast. We detected two polypeptides that most probably correspond to core subunits of yeast RNA-polymerase II in the coding region of transketolase gene (TKL2). Several non-histone proteins were also detected which bind to the upstream region of TKL2 gene, and to the intergenic spacer between cahnodulin (CMD1) and β-mannosyl transferase (ALG1) genes.

KW - DNA-binding protein

KW - DNA-protein crosslinking

KW - Saccharomyces cerevisiae

KW - UV-light

UR - https://www.scopus.com/pages/publications/0030047479

U2 - 10.1016/0014-5793(96)00091-9

DO - 10.1016/0014-5793(96)00091-9

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VL - 381

SP - 103

EP - 105

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Mapping of DNA-binding proteins along the yeast genome by UV-induced DNA-protein crosslinking

Abstract

Keywords

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