Manipulation of individual carbon nanotubes by reconstructing the intracellular transport of a living cell

Cerasela Zoica Dinu, Shyam Sundhar Bale, Douglas B. Chrisey, Jonathan S. Dordick

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The use of the cellular motor protein kinesin and the synthetically reconstituted microtubule for active transport of multiwalled carbon nanotubes (MWNTs) in synthetic, non-physiological environments, was studied. Oxidized nanotubes were prepared by adding H2SO4 and HNO3 to MWNTs. The suspension was sonicated for 3 h at room temperature. Subsequently, the acid mixture was diluted in water and the solution was filtered through a 0.2mm filter membrane. Protein loading was measured using the standard BCA assay. The absorbance at 562nm was measured on a UV-Vis spectrophotometer. The results obtained clearly show that the MWNTs are attached to microtubules and that although coated with MWNTs, the microtubules were propelled by the kinesin motors in the presence of ATP. Two geometries were observed, including different microtubules connected by the same nanotube or microtubules carrying more than one nanotube.

Original languageEnglish
Pages (from-to)1182-1186
Number of pages5
JournalAdvanced Materials
Volume21
Issue number10-11
DOIs
StatePublished - 20 Mar 2009
Externally publishedYes

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