@article{a84f77354ee047578540561d890c5ba9,
title = "Manipulating and tracking single hepatocyte behavior during mouse liver regeneration by performing hydrodynamic tail vein injection",
abstract = "Studies to identify genes relevant to mammalian hepatocyte biology in vivo are largely carried out using germline genetic-engineering approaches, which can be costly and time-consuming. We describe hydrodynamic tail vein injection as an alternative approach to introduce genetic elements into hepatocytes. Transfected hepatocytes can then be traced with a GFP reporter enabling the use of immunohistochemistry and FACS sorting to examine the changes in hepatocyte gene expression and proliferation during liver regeneration induced by 2/3 partial hepatectomy (PH). For complete details on the use and execution of this protocol, please refer to Wang et al. (2019).",
keywords = "Cell Biology, Flow Cytometry/Mass Cytometry, Metabolism, Microscopy, Model Organisms, Molecular Biology",
author = "Shuang Wang and {Ruiz de Galarreta}, Marina and Sadler, {Kirsten C.} and Amaia Lujambio",
note = "Funding Information: Funding was received from NIH/NIDDK (5R01DK080789 to K.C.S. and R37CA230636 to A.L.), NYUAD Investigator Funding (to K.C.S.), and the American Gastroenterological Association (AGA2020-13-03 to S.W.). This work is supported by the Flow Cytometry CORE and Division of Liver Diseases research facilities at Mount Sinai. The Tisch Cancer Institute and related research facilities are supported by P30 CA196521. S.W. and K.C.S. conceived of and designed the experiments. S.W. M.R.G. and A.L. carried out the experiments. K.C.S. and A.L. supervised the studies. S.W. wrote the manuscript with input from M.R.G. K.C.S. and A.L. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = jun,
day = "18",
doi = "10.1016/j.xpro.2021.100440",
language = "English",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}