Maintenance of membrane integrity and permeability depends on a patched-related protein in Caenorhabditis elegans

Myung Kyu Choi, Sangwon Son, Mingi Hong, Min Sung Choi, Jae Young Kwon, Junho Lee

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Membrane integrity is critical for cell survival, defects of which cause pathological symptoms such as metabolic diseases. In this study, we used ethanol sensitivity of the nematode Caenorhabditis elegans to identify genetic factors involved in membrane integrity. In C. elegans, acute exposure to a high concentration (7% v/v) of ethanol changes membrane permeability, as measured by propidium iodide staining, and causes paralysis. We used the timing of complete paralysis as an indicator for alteration of membrane integrity in our genetic screen, and identified ptr-6 as a gene that confers ethanol resistance when mutated. PTR-6 is a patched-related protein and contains a sterol sensing domain. Inhibition of two PTR-encoding genes, ptr-15 and ptr-23, and mboa-1, encoding an Acyl Co-A: cholesterol acyltransferase homolog, restored ethanol sensitivity of the ptr-6 mutant, suggesting that these ptr genes and mboa-1 are involved in the maintenance of membrane integrity and permeability. Our results suggest that C. elegans canbeusedasamodelsystem to identify factors involved in metabolic diseases and to screen for therapeutic drugs.

Original languageEnglish
Pages (from-to)1411-1420
Number of pages10
JournalGenetics
Volume202
Issue number4
DOIs
StatePublished - Apr 2016

Keywords

  • Alcohol
  • Caenorhabditis elegans
  • Membrane integrity
  • Patched-related

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