TY - JOUR
T1 - Magnesium-dependent sphingomyelinase
AU - Gatt, Shimon
N1 - Funding Information:
ACKNOWLEDGEMENTS: This work was supported in part by NM grant NS02967. The expert technical assistance of Mr. Avigdor Herzl is acknowledged. Thanks are due to Dr. A. Loyter for making his manuscript on the sphingomyelinase of chicken erythrocytes available to us prior to its publication. To Dr. Stoffel for a sample of the dimethyl precursor of sphingomyelin and for details of the method of its methylation. To Drs. P. Greenzaid and M. Heller for a sample of 3H-choline-labelled lecithin and Dr. D. Shapiro for synthetic ceramide .
PY - 1976/1/12
Y1 - 1976/1/12
N2 - An enzyme which requires divalent metals and hydrolyses sphingomyelin to ceramide and phosphorylcholine is present in rat and human brain and practically absent from other organs. The greatest activity is associated with the microsomal fraction. It had an optimal pH at about 7.4, required magnesium or manganese ions and was completely inhibited by EDTA. Triton X-100 was required for optimal activity and this detergent could also be used to partly solubilize the enzyme from rat brain microsomes. Lecithin was hydrolyzed at only 2% of the corresponding rate of hydrolysis of sphingomyelin.
AB - An enzyme which requires divalent metals and hydrolyses sphingomyelin to ceramide and phosphorylcholine is present in rat and human brain and practically absent from other organs. The greatest activity is associated with the microsomal fraction. It had an optimal pH at about 7.4, required magnesium or manganese ions and was completely inhibited by EDTA. Triton X-100 was required for optimal activity and this detergent could also be used to partly solubilize the enzyme from rat brain microsomes. Lecithin was hydrolyzed at only 2% of the corresponding rate of hydrolysis of sphingomyelin.
UR - http://www.scopus.com/inward/record.url?scp=0017282664&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(76)90034-6
DO - 10.1016/0006-291X(76)90034-6
M3 - Article
C2 - 174564
AN - SCOPUS:0017282664
SN - 0006-291X
VL - 68
SP - 235
EP - 241
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -