TY - JOUR
T1 - Macrophage-derived chemokine expression in classical Hodgkin's lymphoma
T2 - Application of tissue microarrays
AU - Hedvat, Cyrus V.
AU - Jaffe, Elaine S.
AU - Qin, Jing
AU - Filippa, Daniel A.
AU - Cordon-Cardo, Carlos
AU - Tosato, Giovanna
AU - Nimer, Stephen D.
AU - Teruya-Feldstein, Julie
N1 - Funding Information:
Copyright © 2001 by The United States and Canadian Academy of Pathology, Inc. VOL. 14, NO. 12, P. 1270, 2001 Printed in the U.S.A. Date of acceptance: September 5, 2001. This work was presented in part at the 90th Annual Meeting of the United States and Canadian Academy of Pathology, March 3–9, 2001, in Atlanta, Georgia. Supported in part by grants from the Norman and Rosita Winston Foundation (CVH) and the Sports for a Cure Foundation (CVH, SDN). Address reprint requests to: Julie Teruya-Feldstein, Department of Pathology, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; e-mail: [email protected]; fax: 212-717-3203.
PY - 2001
Y1 - 2001
N2 - Hodgkin's disease (HD) is a lymphoid malignancy characterized by the presence of Reed-Sternberg (RS) and Hodgkin's cells in a background of mixed inflammatory cells and stromal reaction. Studies have documented that HD is a neoplasm associated with abnormal cytokine and chemokine production. To define the expression of macrophagederived chemokine (MDC) in HD, 57 cases (18 lymphocyte predominant, 11 mixed cellularity, 28 nodular sclerosis) were stained for MDC by immunohistochemistry and compared with reactive lymph nodes as controls. MDC was expressed by RS cells in classical HD (CHD) and showed a distinct cytoplasmic and Golgi localization. Accumulating evidence suggests that lymphocyte-predominant HD (LPHD) represents an entity distinct from CHD, with different biological properties and clinical course. On the basis of the high level of MDC staining alone, CHD could be distinguished from LPHD (P < .001), which showed only faint staining of scattered histiocytes similar to control tissues. CHD cases with high MDC mRNA levels showed high levels of MDC protein expression by immunohistochemistry (P < .001) and significant eosinophil infiltration, suggesting that MDC may represent another molecule that plays a critical role in eosinophil recruitment. We also analyzed 102 cases of non-Hodgkin's lymphoma and normal spleen, lymph node, and thymic tissue. High levels of MDC expression were specific to CHD cases because only low levels of MDC were observed in a minor subset of LPHD, NHL or normal lymphoid tissues.
AB - Hodgkin's disease (HD) is a lymphoid malignancy characterized by the presence of Reed-Sternberg (RS) and Hodgkin's cells in a background of mixed inflammatory cells and stromal reaction. Studies have documented that HD is a neoplasm associated with abnormal cytokine and chemokine production. To define the expression of macrophagederived chemokine (MDC) in HD, 57 cases (18 lymphocyte predominant, 11 mixed cellularity, 28 nodular sclerosis) were stained for MDC by immunohistochemistry and compared with reactive lymph nodes as controls. MDC was expressed by RS cells in classical HD (CHD) and showed a distinct cytoplasmic and Golgi localization. Accumulating evidence suggests that lymphocyte-predominant HD (LPHD) represents an entity distinct from CHD, with different biological properties and clinical course. On the basis of the high level of MDC staining alone, CHD could be distinguished from LPHD (P < .001), which showed only faint staining of scattered histiocytes similar to control tissues. CHD cases with high MDC mRNA levels showed high levels of MDC protein expression by immunohistochemistry (P < .001) and significant eosinophil infiltration, suggesting that MDC may represent another molecule that plays a critical role in eosinophil recruitment. We also analyzed 102 cases of non-Hodgkin's lymphoma and normal spleen, lymph node, and thymic tissue. High levels of MDC expression were specific to CHD cases because only low levels of MDC were observed in a minor subset of LPHD, NHL or normal lymphoid tissues.
KW - Chemokine
KW - Hodgkin's lymphoma
KW - Lymphoma
KW - MDC
KW - Tissue microarray
UR - http://www.scopus.com/inward/record.url?scp=0035215993&partnerID=8YFLogxK
U2 - 10.1038/modpathol.3880473
DO - 10.1038/modpathol.3880473
M3 - Article
C2 - 11743050
AN - SCOPUS:0035215993
SN - 0893-3952
VL - 14
SP - 1270
EP - 1276
JO - Modern Pathology
JF - Modern Pathology
IS - 12
ER -