TY - JOUR
T1 - Lysosomes and secretory granules in the normal and autotransplanted salamander pars distalis
AU - Masur, Sandra Kazahn
AU - Holtzman, Eric
N1 - Funding Information:
In a previous paper (Masur, 1968) we described the changes in the amphibian pituitary gland after transplantation. At all stages, bodies morphologically resembling lysosomes were noted (Novikoff, 1963; de Duve and Wattiaux, 1966). Lysosomes are important in the response of many cells to altered conditions (de Duve and Wat-tiaux, 1966; Novikoff, 1963; Weissman, 1965). Recent work on mammalian glands (Smith and Farquhar, 1966; Szabo et al., 1967; Holtzman and Dominit,z, 1968) has suggested roles for these organelles in secretory cells, including involvement in the secretory cycle. Thus, it seemed worth-while to use cytochemical methods to aid in identifying the lysosomcs of the amphibian pituitary and to follow their behavior in transplants. As a cytochcmical criterion, the presence of demonstrable acid phos-phataecl in :I membrane-tlclimited body has ‘This study was supported by NSF Grant GB6950 t,o Eric Holtzman. A preliminary report of this work was given at the 1968 meeting of the American Society for Cell Biology. (Mxrnr el nl.. 1968). ’ Present address : Depart,ment of Physiology, Mt. Sinai School of Medicine, The City University of New York. New York.
PY - 1969/2
Y1 - 1969/2
N2 - Normal and autotransplanted salamander pituitary glands were studied by light and electron microscopy. Frozen sections of glutaraldehyde-fixed material were incubated for demonstration of acid phosphatase, a "marker" enzyme for lysosomes. Acid phosphatase is demonstrable in the agranular membrane systems and vesicles associated with the Golgi apparatus, in newly formed secretory granules, and in lysosomes of both the normal and autotransplanted par distalis. Among the lysosomes, autophagic vacuoles containing secretory granules are more common in the hypersecretory cell types of the autotransplanted pituitary than in the normal gland.
AB - Normal and autotransplanted salamander pituitary glands were studied by light and electron microscopy. Frozen sections of glutaraldehyde-fixed material were incubated for demonstration of acid phosphatase, a "marker" enzyme for lysosomes. Acid phosphatase is demonstrable in the agranular membrane systems and vesicles associated with the Golgi apparatus, in newly formed secretory granules, and in lysosomes of both the normal and autotransplanted par distalis. Among the lysosomes, autophagic vacuoles containing secretory granules are more common in the hypersecretory cell types of the autotransplanted pituitary than in the normal gland.
UR - https://www.scopus.com/pages/publications/0014466607
U2 - 10.1016/0016-6480(69)90134-8
DO - 10.1016/0016-6480(69)90134-8
M3 - Article
C2 - 5765444
AN - SCOPUS:0014466607
SN - 0016-6480
VL - 12
SP - 33
EP - 39
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 1
ER -