Low-input fluidized-bed enabled ChIP-seq device with parallel units for analysis of histone modifications

Travis W. Murphy, Sai Ma, Chang Lu

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Epigenetic changes, such as histone modifications, have been implicated in a number of different disorders, such as heart disease, cancer, and autism. Therefore, it is imperative that we develop a methodology to effectively study epigenetic modifications in an efficient and rapid manner. Furthermore, a variety of histone modifications are routinely involved in gene regulation. In this paper we demonstrate LIFE-ChIP (Low Input Fluidized-bed Enabled Chromatin Immunoprecipitation), an automated and high throughput microfluidic platform capable of producing multiple sets of high quality ChIP-seq data in as little as 1 hour with as few as 50 cells per reaction.

Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages189-190
Number of pages2
ISBN (Electronic)9780692941836
StatePublished - 2020
Externally publishedYes
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: 22 Oct 201726 Oct 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Country/TerritoryUnited States
CitySavannah
Period22/10/1726/10/17

Keywords

  • Chromatin immunoprecipitation
  • Epigenomics
  • High throughput

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