TY - JOUR
T1 - Low-flow perfusion preservation versus static preservation for isolated rat heart
T2 - Effects on recovery of myocardial function
AU - Li, L. B.
AU - Ma, L.
AU - Su, C.
AU - Zhang, T.
AU - Gao, C. Q.
N1 - Funding Information:
Supported by grants from the National Nature Sciences Foundation of China ( 30971182 , 30670823 ).
PY - 2013/3
Y1 - 2013/3
N2 - Background: Clinically, donor hearts cannot be preserved for >6 hours between explantation and recipient implantation. A better approach is needed to preserve donor hearts for a longer time. We tested whether low-flow perfusion (LFP) could satisfactorily preserve isolated rat hearts with histidine-tryptophan-ketoglutarate (HTK) solution or Fuwai modified (FWM) solution. Methods: We divided 32 male Sprague-Dawley rats randomly into 4 groups (n = 8): H1, H2, F1, and F2. The Langendorff heart model immersed isolated hearts in the H1 and F1 groups in HTK or FWM solution for 8 hours at 4°C. Isolated hearts in the H2 and F2 groups were low-flow perfused with HTK solution and FWM solution for 8 hours at a pressure of 10 cmH2O at 4°C. After 60 minutes reperfusion, we measured recovery of cardiac function, myocardial water content, and leakage of myocardial enzymes. Results: After reperfusion, no cardiac rebeating was observed among F1 group hearts; in addition, they showed significantly higher myocardial water content and lactate dehydrogenase leakage compared with the other 3 groups (P <.05). The recovery rates of cardiac function among H2 hearts were better than the other 3 groups (P <.05); their myocardial water content and enzyme leakage were less than the other 3 groups (P <.05). Conclusions: Hypothermic LFP was better than static storage to preserve isolated rat hearts. HTK solution afforded better myocardial protection than FWM.
AB - Background: Clinically, donor hearts cannot be preserved for >6 hours between explantation and recipient implantation. A better approach is needed to preserve donor hearts for a longer time. We tested whether low-flow perfusion (LFP) could satisfactorily preserve isolated rat hearts with histidine-tryptophan-ketoglutarate (HTK) solution or Fuwai modified (FWM) solution. Methods: We divided 32 male Sprague-Dawley rats randomly into 4 groups (n = 8): H1, H2, F1, and F2. The Langendorff heart model immersed isolated hearts in the H1 and F1 groups in HTK or FWM solution for 8 hours at 4°C. Isolated hearts in the H2 and F2 groups were low-flow perfused with HTK solution and FWM solution for 8 hours at a pressure of 10 cmH2O at 4°C. After 60 minutes reperfusion, we measured recovery of cardiac function, myocardial water content, and leakage of myocardial enzymes. Results: After reperfusion, no cardiac rebeating was observed among F1 group hearts; in addition, they showed significantly higher myocardial water content and lactate dehydrogenase leakage compared with the other 3 groups (P <.05). The recovery rates of cardiac function among H2 hearts were better than the other 3 groups (P <.05); their myocardial water content and enzyme leakage were less than the other 3 groups (P <.05). Conclusions: Hypothermic LFP was better than static storage to preserve isolated rat hearts. HTK solution afforded better myocardial protection than FWM.
UR - https://www.scopus.com/pages/publications/84875158734
U2 - 10.1016/j.transproceed.2012.08.022
DO - 10.1016/j.transproceed.2012.08.022
M3 - Article
C2 - 23498788
AN - SCOPUS:84875158734
SN - 0041-1345
VL - 45
SP - 523
EP - 527
JO - Transplantation Proceedings
JF - Transplantation Proceedings
IS - 2
ER -