TY - GEN
T1 - Low calcium and high fructose diet diminish the quality of circumferential long-bone growth
AU - Williams, E. A.J.
AU - Douard, V.
AU - Bajaj, D.
AU - Sabbagh, Y.
AU - Ferraris, R.
AU - Fritton, J. C.
PY - 2013
Y1 - 2013
N2 - High fructose (HF) intake is a public health problem. However, little is known about effects on bone quality during growth. Greater data exists for low calcium (Ca2+). We fed low-Ca (0.02%) and calorie-equivalent glucose and fructose diets (43%) to male, 3-wk-old, B6 mice for 5 wks. Four groups (n=9/group) were fed combinations of normal/low Ca and glucose/fructose. Weight and long-bone lengths were not affected. Consistent with literature, low-Ca diet resulted in less stiff and strong (∼40%; p65% (p20%, p<0.01) long bone and increased toughness (energy absorption) of longbone mid-shaft, measured by 3-point bending; post-yield displacement was increased in low Ca by >65% (p<0.05). By histomorphometry, low Ca resulted in decreased (>20%, p<0.05) cortical width, partially explaining the bending results. Only the glucose-fed group exhibited compensation to maintain outside circumference and diameter by increased periosteal bone formation rate (p.BFR); 3-fold greater vs. each of the other 3 groups (p<0.05). One plausible mechanism for poor circumferential growth involves bone in a feedback loop and is suggested by decreased intestinal Ca transport and Ca transporter protein in mucosa. The fructose diet completely prevented both adaptive increases in Ca transport, and 1α-hydroxylase-mediated synthesis of 1,25(OH)2D3 levels.
AB - High fructose (HF) intake is a public health problem. However, little is known about effects on bone quality during growth. Greater data exists for low calcium (Ca2+). We fed low-Ca (0.02%) and calorie-equivalent glucose and fructose diets (43%) to male, 3-wk-old, B6 mice for 5 wks. Four groups (n=9/group) were fed combinations of normal/low Ca and glucose/fructose. Weight and long-bone lengths were not affected. Consistent with literature, low-Ca diet resulted in less stiff and strong (∼40%; p65% (p20%, p<0.01) long bone and increased toughness (energy absorption) of longbone mid-shaft, measured by 3-point bending; post-yield displacement was increased in low Ca by >65% (p<0.05). By histomorphometry, low Ca resulted in decreased (>20%, p<0.05) cortical width, partially explaining the bending results. Only the glucose-fed group exhibited compensation to maintain outside circumference and diameter by increased periosteal bone formation rate (p.BFR); 3-fold greater vs. each of the other 3 groups (p<0.05). One plausible mechanism for poor circumferential growth involves bone in a feedback loop and is suggested by decreased intestinal Ca transport and Ca transporter protein in mucosa. The fructose diet completely prevented both adaptive increases in Ca transport, and 1α-hydroxylase-mediated synthesis of 1,25(OH)2D3 levels.
KW - Ca
KW - bone formation
KW - fructose
KW - mechanical properties of bone
UR - https://www.scopus.com/pages/publications/84886995511
U2 - 10.1109/NEBEC.2013.109
DO - 10.1109/NEBEC.2013.109
M3 - Conference contribution
AN - SCOPUS:84886995511
SN - 9780769549644
T3 - Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
SP - 227
EP - 228
BT - Proceedings - 39th Annual Northeast Bioengineering Conference, NEBEC 2013
T2 - 39th Annual Northeast Bioengineering Conference, NEBEC 2013
Y2 - 5 April 2013 through 7 April 2013
ER -