TY - JOUR
T1 - Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice
AU - Swiss IBD Cohort Investigators
AU - Wuggenig, Philipp
AU - Kaya, Berna
AU - Melhem, Hassan
AU - Ayata, C. Korcan
AU - Abdelrahman, Karim
AU - Ademi, Gentiana
AU - Aepli, Patrick
AU - Anderegg, Claudia
AU - Antonino, Anca Teodora
AU - Archanioti, Eva
AU - Arrigoni, Eviano
AU - de Jong, Diana Bakker
AU - Balsiger, Bruno
AU - Bastürk, Polat
AU - Bauerfeind, Peter
AU - Becocci, Andrea
AU - Belli, Dominique
AU - Bengoa, José M.
AU - Biedermann, Luc
AU - Binek, Janek
AU - Blattmann, Mirjam
AU - Boehm, Stephan
AU - Boldanova, Tujana
AU - Borovicka, Jan
AU - Braegger, Christian P.
AU - Brand, Stephan
AU - Brügger, Lukas
AU - Brunner, Simon
AU - Bühr, Patrick
AU - Burk, Sabine
AU - Burnand, Bernard
AU - Burri, Emanuel
AU - Buyse, Sophie
AU - Cao, Dahlia Thao
AU - Carstens, Ove
AU - Criblez, Dominique H.
AU - Cunningham, Sophie
AU - D’Angelo, Fabrizia
AU - de Saussure, Philippe
AU - Degen, Lukas
AU - Delarive, Joakim
AU - Doerig, Christopher
AU - Dora, Barbara
AU - Drerup, Susan
AU - Egger, Mara
AU - El-Wafa, Ali
AU - Engelmann, Matthias
AU - Ezri, Jessica
AU - Felley, Christian
AU - Sauter, Bernhard
N1 - Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Comprehensive development is critical for gut macrophages being essential for the intestinal immune system. However, the underlying mechanisms of macrophage development in the colon remain elusive. To investigate the function of branched-chain amino acids in the development of gut macrophages, an inducible knock-out mouse model for the branched-chain amino acid transporter CD98hc in CX3CR1+ macrophages was generated. The relatively selective deletion of CD98hc in macrophage populations leads to attenuated severity of chemically-induced colitis that we assessed by clinical, endoscopic, and histological scoring. Single-cell RNA sequencing of colonic lamina propria macrophages revealed that conditional deletion of CD98hc alters the “monocyte waterfall”-development to MHC II+ macrophages. The change in the macrophage development after deletion of CD98hc is associated with increased apoptotic gene expression. Our results show that CD98hc deletion changes the development of colonic macrophages.
AB - Comprehensive development is critical for gut macrophages being essential for the intestinal immune system. However, the underlying mechanisms of macrophage development in the colon remain elusive. To investigate the function of branched-chain amino acids in the development of gut macrophages, an inducible knock-out mouse model for the branched-chain amino acid transporter CD98hc in CX3CR1+ macrophages was generated. The relatively selective deletion of CD98hc in macrophage populations leads to attenuated severity of chemically-induced colitis that we assessed by clinical, endoscopic, and histological scoring. Single-cell RNA sequencing of colonic lamina propria macrophages revealed that conditional deletion of CD98hc alters the “monocyte waterfall”-development to MHC II+ macrophages. The change in the macrophage development after deletion of CD98hc is associated with increased apoptotic gene expression. Our results show that CD98hc deletion changes the development of colonic macrophages.
UR - http://www.scopus.com/inward/record.url?scp=85082108238&partnerID=8YFLogxK
U2 - 10.1038/s42003-020-0842-3
DO - 10.1038/s42003-020-0842-3
M3 - Article
C2 - 32188932
AN - SCOPUS:85082108238
SN - 2399-3642
VL - 3
JO - Communications Biology
JF - Communications Biology
IS - 1
M1 - 130
ER -