TY - JOUR
T1 - Longitudinal Study of Apoptosis in Chronic Uremic Patients
AU - D'Intini, Vincenzo
AU - Bordoni, Valeria
AU - Fortunato, Antonio
AU - Galloni, Elisabetta
AU - Carta, Mariarita
AU - Galli, Francesco
AU - Bolgan, Irene
AU - Inguaggiato, Paola
AU - Poulin, Sonya
AU - Bonello, Monica
AU - Tetta, Ciro
AU - Levin, Nathan
AU - Ronco, Claudio
PY - 2003/11
Y1 - 2003/11
N2 - Uremia is associated with a state of immune dysfunction, increasing infection and malignancy rates. Dysregulation of homeostasis may be directly related to abnormal apoptosis regulation, a process which is crucial for the maintenance of the biologic system. Abnormal apoptosis rates (ARs) have been reported in the literature. We performed a longitudinal study over a 10-week period in three groups of uremic subjects - hemodialysis (HD), peritoneal dialysis (PD), and predialysis chronic renal failure (CRF). Our results showed that ARs were consistent over the observed period. Monocytes extracted from HD and CRF subjects had higher ARs compared to PD and controls (HD: 26.06 ± 8.82; CRF: 26.96 ± 12.81; PD: 14.77 ± 5.87; C: 11.42 ± 4.60) when placed in culture medium. The plasma of HD and CRF subjects when incubated with U937 cells had a stronger apoptogenic potential compared with PD and controls (HD: 26.08 ± 11.39; CRF: 24.87 ± 9.07; PD: 12.13 ± 4.51; C: 11.69 ± 4.02). Inflammatory markers (C-reactive protein [CRP], procalcitonin) and cytokines (interleukin [IL]-β, IL-2, IL-10) had a generally poor correlation except for tumor necrosis factor (TNF)-α (p < 0.001). The phagocytic ability of U937 cells when incubated with the various plasma demonstrated impaired response in the HD and CRF subjects (HD: 27.56 ± 6.67; CRF: 30.24 ± 9.08; PD: 36.55 ± 9.80; C: 40.04 ± 6.98). These results suggest continuous renal purification, such as in continuous ambulatory peritoneal dialysis (CAPD), may have advantages over intermittent therapies in regulating apoptosis and maintaining biologic function and homeostasis.
AB - Uremia is associated with a state of immune dysfunction, increasing infection and malignancy rates. Dysregulation of homeostasis may be directly related to abnormal apoptosis regulation, a process which is crucial for the maintenance of the biologic system. Abnormal apoptosis rates (ARs) have been reported in the literature. We performed a longitudinal study over a 10-week period in three groups of uremic subjects - hemodialysis (HD), peritoneal dialysis (PD), and predialysis chronic renal failure (CRF). Our results showed that ARs were consistent over the observed period. Monocytes extracted from HD and CRF subjects had higher ARs compared to PD and controls (HD: 26.06 ± 8.82; CRF: 26.96 ± 12.81; PD: 14.77 ± 5.87; C: 11.42 ± 4.60) when placed in culture medium. The plasma of HD and CRF subjects when incubated with U937 cells had a stronger apoptogenic potential compared with PD and controls (HD: 26.08 ± 11.39; CRF: 24.87 ± 9.07; PD: 12.13 ± 4.51; C: 11.69 ± 4.02). Inflammatory markers (C-reactive protein [CRP], procalcitonin) and cytokines (interleukin [IL]-β, IL-2, IL-10) had a generally poor correlation except for tumor necrosis factor (TNF)-α (p < 0.001). The phagocytic ability of U937 cells when incubated with the various plasma demonstrated impaired response in the HD and CRF subjects (HD: 27.56 ± 6.67; CRF: 30.24 ± 9.08; PD: 36.55 ± 9.80; C: 40.04 ± 6.98). These results suggest continuous renal purification, such as in continuous ambulatory peritoneal dialysis (CAPD), may have advantages over intermittent therapies in regulating apoptosis and maintaining biologic function and homeostasis.
UR - http://www.scopus.com/inward/record.url?scp=10744231616&partnerID=8YFLogxK
U2 - 10.1046/j.1525-139X.2003.16101.x
DO - 10.1046/j.1525-139X.2003.16101.x
M3 - Article
C2 - 14629608
AN - SCOPUS:10744231616
SN - 0894-0959
VL - 16
SP - 467
EP - 473
JO - Seminars in Dialysis
JF - Seminars in Dialysis
IS - 6
ER -