Long-term storage of lipid-like nanoparticles for mRNA delivery

Pengxuan Zhao; Xucheng Hou; Jingyue Yan; Shi Du; Yonger Xue; Wenqing Li; Guangya Xiang; Yizhou Dong

doi:10.1016/j.bioactmat.2020.03.001

Long-term storage of lipid-like nanoparticles for mRNA delivery

Pengxuan Zhao, Xucheng Hou, Jingyue Yan, Shi Du, Yonger Xue, Wenqing Li, Guangya Xiang, Yizhou Dong

Research output: Contribution to journal › Article › peer-review

173 Scopus citations

Abstract

Lipid-like nanoparticles (LLNs) have been extensively explored for messenger RNA (mRNA) delivery in various biomedical applications. However, the long-term storage of these nanoparticles is still a challenge for their clinical translation. In this study, we investigated a series of conditions for the long-term storage of LLNs with encapsulation of mRNA. We evaluated the stability of LLNs with different concentrations of cryoprotectants (sucrose, trehalose or mannitol) under the conditions of freezing or lyophilization processes. Through in vitro and in vivo mRNA delivery studies, we identified the optimal storage condition, and found that the addition with 5% (w/v) sucrose or trehalose to LLNs could remain their mRNA delivery efficiency for at least three months in the liquid nitrogen storage condition.

Original language	English
Pages (from-to)	358-363
Number of pages	6
Journal	Bioactive Materials
Volume	5
Issue number	2
DOIs	https://doi.org/10.1016/j.bioactmat.2020.03.001
State	Published - Jun 2020
Externally published	Yes

Keywords

Lipid-like nanoparticles
Long-term storage
mRNA delivery

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10.1016/j.bioactmat.2020.03.001

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title = "Long-term storage of lipid-like nanoparticles for mRNA delivery",

abstract = "Lipid-like nanoparticles (LLNs) have been extensively explored for messenger RNA (mRNA) delivery in various biomedical applications. However, the long-term storage of these nanoparticles is still a challenge for their clinical translation. In this study, we investigated a series of conditions for the long-term storage of LLNs with encapsulation of mRNA. We evaluated the stability of LLNs with different concentrations of cryoprotectants (sucrose, trehalose or mannitol) under the conditions of freezing or lyophilization processes. Through in vitro and in vivo mRNA delivery studies, we identified the optimal storage condition, and found that the addition with 5% (w/v) sucrose or trehalose to LLNs could remain their mRNA delivery efficiency for at least three months in the liquid nitrogen storage condition.",

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author = "Pengxuan Zhao and Xucheng Hou and Jingyue Yan and Shi Du and Yonger Xue and Wenqing Li and Guangya Xiang and Yizhou Dong",

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doi = "10.1016/j.bioactmat.2020.03.001",

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T1 - Long-term storage of lipid-like nanoparticles for mRNA delivery

AU - Zhao, Pengxuan

AU - Hou, Xucheng

AU - Yan, Jingyue

AU - Du, Shi

AU - Xue, Yonger

AU - Li, Wenqing

AU - Xiang, Guangya

AU - Dong, Yizhou

PY - 2020/6

Y1 - 2020/6

N2 - Lipid-like nanoparticles (LLNs) have been extensively explored for messenger RNA (mRNA) delivery in various biomedical applications. However, the long-term storage of these nanoparticles is still a challenge for their clinical translation. In this study, we investigated a series of conditions for the long-term storage of LLNs with encapsulation of mRNA. We evaluated the stability of LLNs with different concentrations of cryoprotectants (sucrose, trehalose or mannitol) under the conditions of freezing or lyophilization processes. Through in vitro and in vivo mRNA delivery studies, we identified the optimal storage condition, and found that the addition with 5% (w/v) sucrose or trehalose to LLNs could remain their mRNA delivery efficiency for at least three months in the liquid nitrogen storage condition.

AB - Lipid-like nanoparticles (LLNs) have been extensively explored for messenger RNA (mRNA) delivery in various biomedical applications. However, the long-term storage of these nanoparticles is still a challenge for their clinical translation. In this study, we investigated a series of conditions for the long-term storage of LLNs with encapsulation of mRNA. We evaluated the stability of LLNs with different concentrations of cryoprotectants (sucrose, trehalose or mannitol) under the conditions of freezing or lyophilization processes. Through in vitro and in vivo mRNA delivery studies, we identified the optimal storage condition, and found that the addition with 5% (w/v) sucrose or trehalose to LLNs could remain their mRNA delivery efficiency for at least three months in the liquid nitrogen storage condition.

KW - Lipid-like nanoparticles

KW - Long-term storage

KW - mRNA delivery

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Long-term storage of lipid-like nanoparticles for mRNA delivery

Abstract

Keywords

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