Long-term culture and passage of human fetal liver cells that synthesize albumin

Mary Ann Sells; Jonathon Chernoff; Arturo Cerda; Charles Bowers; David A. Shafritz; Nathan Kase; Judith K. Christman; George Acs

doi:10.1007/BF02620932

Long-term culture and passage of human fetal liver cells that synthesize albumin

Mary Ann Sells, Jonathon Chernoff, Arturo Cerda, Charles Bowers, David A. Shafritz, Nathan Kase, Judith K. Christman, George Acs

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Long-term cultures of hepatocytes were established from livers of human fetuses obtained by abortion at 18 to 23 wk of gestation. Cells obtained by collagenase dissociation of liver were maintained in defined serum-free medium on a substratum of positively charged plastic. Under these conditions, the cells divide and form a confluent monolayer. After multiple passages over a period of 3 mo., the cells retained an epithelioid morphology and continued to synthesize and secrete albumin.

Original language	English
Pages (from-to)	216-220
Number of pages	5
Journal	In Vitro Cellular & Developmental Biology
Volume	21
Issue number	4
DOIs	https://doi.org/10.1007/BF02620932
State	Published - Apr 1985
Externally published	Yes

Keywords

albumin production
cultured human liver cells
passage

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10.1007/BF02620932

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title = "Long-term culture and passage of human fetal liver cells that synthesize albumin",

abstract = "Long-term cultures of hepatocytes were established from livers of human fetuses obtained by abortion at 18 to 23 wk of gestation. Cells obtained by collagenase dissociation of liver were maintained in defined serum-free medium on a substratum of positively charged plastic. Under these conditions, the cells divide and form a confluent monolayer. After multiple passages over a period of 3 mo., the cells retained an epithelioid morphology and continued to synthesize and secrete albumin.",

keywords = "albumin production, cultured human liver cells, passage",

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AU - Sells, Mary Ann

AU - Chernoff, Jonathon

AU - Cerda, Arturo

AU - Bowers, Charles

AU - Shafritz, David A.

AU - Kase, Nathan

AU - Christman, Judith K.

AU - Acs, George

PY - 1985/4

Y1 - 1985/4

N2 - Long-term cultures of hepatocytes were established from livers of human fetuses obtained by abortion at 18 to 23 wk of gestation. Cells obtained by collagenase dissociation of liver were maintained in defined serum-free medium on a substratum of positively charged plastic. Under these conditions, the cells divide and form a confluent monolayer. After multiple passages over a period of 3 mo., the cells retained an epithelioid morphology and continued to synthesize and secrete albumin.

AB - Long-term cultures of hepatocytes were established from livers of human fetuses obtained by abortion at 18 to 23 wk of gestation. Cells obtained by collagenase dissociation of liver were maintained in defined serum-free medium on a substratum of positively charged plastic. Under these conditions, the cells divide and form a confluent monolayer. After multiple passages over a period of 3 mo., the cells retained an epithelioid morphology and continued to synthesize and secrete albumin.

KW - albumin production

KW - cultured human liver cells

KW - passage

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JF - In Vitro Cellular & Developmental Biology

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ER -

Long-term culture and passage of human fetal liver cells that synthesize albumin

Abstract

Keywords

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