Long-range genomic interactions epigenetically regulate the expression of a cytokine receptor

Chrysoula Deligianni, Charalampos G. Spilianakis

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Current research on the cytokine-mediated signalling towards the polarization and differentiation of a T-helper cell lineage lacks mechanistic insights on the transcriptional regulation of cytokine receptor genes. Here, we propose a new mechanism for the transcriptional regulation of the interferon gamma receptor 1 gene via long-range intrachromosomal interactions with the IfnÎ 3 locus mediated by the protein CTCF. These interactions sustain the monoallelic expression of the differentially methylated IfnÎ 3R1 gene and are persistent on blockade of active transcription. Our findings suggest that regulatory elements for a cytokine gene locus can also positively regulate the transcription of its receptor.

Original languageEnglish
Pages (from-to)819-826
Number of pages8
JournalEMBO Reports
Volume13
Issue number9
DOIs
StatePublished - Sep 2012
Externally publishedYes

Keywords

  • 3C
  • CD4 cells
  • ChIP-loop
  • IFN-g signalling
  • RNA-DNA FISH

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