Location of rRNA genes in three inbred strains of rat and suppression of rat rRNA activity in rat-human somatic cell hybrids

R. Tantravahi; D. A. Miller; G. D'Ancona; C. M. Croce; O. J. Miller

doi:10.1016/0014-4827(79)90368-9

Location of rRNA genes in three inbred strains of rat and suppression of rat rRNA activity in rat-human somatic cell hybrids

R. Tantravahi
, D. A. Miller
, G. D'Ancona
, C. M. Croce
, O. J. Miller

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Nucleolus organizer regions (NORs) of rat chromosomes were stained by the Ag-AS method. The Ag-NORs were found on chromosomes 3, 11 and 12 in the ACI, Wistar Brown and Wistar Lewis inbred strains of rat. The size of the Ag-NOR on each pair of chromosomes varied from strain to strain. Rat-human somatic hybrid cells that retained human and lost some of the rat chromosomes had no Ag-NOR on rat chromosomes 3, 11 or 12. Since NORs can be Ag-stained only if their 18 + 28S rRNA genes are active, the activity of the rat rRNA genes must have been suppressed in the hybrid cells.

Original language	English
Pages (from-to)	387-392
Number of pages	6
Journal	Experimental Cell Research
Volume	119
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(79)90368-9
State	Published - 15 Mar 1979
Externally published	Yes

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@article{ce287b30d4644d8ea13e4b63861c29c7,

title = "Location of rRNA genes in three inbred strains of rat and suppression of rat rRNA activity in rat-human somatic cell hybrids",

abstract = "Nucleolus organizer regions (NORs) of rat chromosomes were stained by the Ag-AS method. The Ag-NORs were found on chromosomes 3, 11 and 12 in the ACI, Wistar Brown and Wistar Lewis inbred strains of rat. The size of the Ag-NOR on each pair of chromosomes varied from strain to strain. Rat-human somatic hybrid cells that retained human and lost some of the rat chromosomes had no Ag-NOR on rat chromosomes 3, 11 or 12. Since NORs can be Ag-stained only if their 18 + 28S rRNA genes are active, the activity of the rat rRNA genes must have been suppressed in the hybrid cells.",

author = "R. Tantravahi and Miller, \{D. A.\} and G. D'Ancona and Croce, \{C. M.\} and Miller, \{O. J.\}",

note = "Funding Information: This work was supported by grants from the ACS (VC-279 to OJM), the NIH (CA12504,G M22966a nd GM25193 to OJM) (CA10815, CA16685, CA20741, CA21069,C A21 124,C A23568a nd GM20700t o CMC), The National Foundation-March of Dimes (l-361 to DAM) (l-522 to CMC) and the NSF (PCM-77-22959 to CMC). CMC is a recipient of a Research Career DevelopmentA ward (CAOO143f)r om the NCI.",

year = "1979",

month = mar,

day = "15",

doi = "10.1016/0014-4827(79)90368-9",

language = "English",

volume = "119",

pages = "387--392",

journal = "Experimental Cell Research",

issn = "0014-4827",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - Location of rRNA genes in three inbred strains of rat and suppression of rat rRNA activity in rat-human somatic cell hybrids

AU - Tantravahi, R.

AU - Miller, D. A.

AU - D'Ancona, G.

AU - Croce, C. M.

AU - Miller, O. J.

N1 - Funding Information: This work was supported by grants from the ACS (VC-279 to OJM), the NIH (CA12504,G M22966a nd GM25193 to OJM) (CA10815, CA16685, CA20741, CA21069,C A21 124,C A23568a nd GM20700t o CMC), The National Foundation-March of Dimes (l-361 to DAM) (l-522 to CMC) and the NSF (PCM-77-22959 to CMC). CMC is a recipient of a Research Career DevelopmentA ward (CAOO143f)r om the NCI.

PY - 1979/3/15

Y1 - 1979/3/15

N2 - Nucleolus organizer regions (NORs) of rat chromosomes were stained by the Ag-AS method. The Ag-NORs were found on chromosomes 3, 11 and 12 in the ACI, Wistar Brown and Wistar Lewis inbred strains of rat. The size of the Ag-NOR on each pair of chromosomes varied from strain to strain. Rat-human somatic hybrid cells that retained human and lost some of the rat chromosomes had no Ag-NOR on rat chromosomes 3, 11 or 12. Since NORs can be Ag-stained only if their 18 + 28S rRNA genes are active, the activity of the rat rRNA genes must have been suppressed in the hybrid cells.

AB - Nucleolus organizer regions (NORs) of rat chromosomes were stained by the Ag-AS method. The Ag-NORs were found on chromosomes 3, 11 and 12 in the ACI, Wistar Brown and Wistar Lewis inbred strains of rat. The size of the Ag-NOR on each pair of chromosomes varied from strain to strain. Rat-human somatic hybrid cells that retained human and lost some of the rat chromosomes had no Ag-NOR on rat chromosomes 3, 11 or 12. Since NORs can be Ag-stained only if their 18 + 28S rRNA genes are active, the activity of the rat rRNA genes must have been suppressed in the hybrid cells.

UR - https://www.scopus.com/pages/publications/0018345724

U2 - 10.1016/0014-4827(79)90368-9

DO - 10.1016/0014-4827(79)90368-9

M3 - Article

C2 - 311737

AN - SCOPUS:0018345724

SN - 0014-4827

VL - 119

SP - 387

EP - 392

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 2

ER -

Location of rRNA genes in three inbred strains of rat and suppression of rat rRNA activity in rat-human somatic cell hybrids

Abstract

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