Locally formed dopamine modulates renal Na-P(i) co-transport through DA1 and DA2 receptors

R. Perrichot, A. Garcia-Ocana, S. Couette, E. Comoy, G. Amiel, G. Friedlander

Research output: Contribution to journalArticlepeer-review

51 Scopus citations


The involvement of dopamine (DA) receptor subtypes in regulation of renal phosphate transport by DA, either exogenous or locally synthesized from L-dihydroxyphenylalanine (L-dopa) was evaluated in opossum kidney (OK) cells with proximal tubular phenotype. DA synthesis from L-dopa by OK cells was abolished by carbidopa and benserazide, two dissimilar inhibitors of aromatic L-amino acid decarboxylase. L-Dopa stimulated cyclic AMP generation and inhibited Na-dependent P(i) uptake, and these effects were abolished by carbidopa and benserazide. The effects of L-dopa or DA on cyclic AMP generation and on Na-P(i) co-transport were mimicked by SKF 38393, a DA1 receptor agonist, and were potentiated by S-sulpiride, a DA2 receptor antagonist. Bromocriptine, a DA2 receptor agonist, blunted in a pertussis toxin-dependent manner parathyroid hormone (PTH)-induced cyclic AMP generation and inhibition of P(i) uptake. In contrast with PTH, neither L-dopa nor DA affected significantly the cytosolic calcium concentration. These results support the involvement of DA1 and DA2 receptors, positively and negatively coupled into adenylate cyclase respectively, in modulation of renal phosphate transport.

Original languageEnglish
Pages (from-to)433-437
Number of pages5
JournalBiochemical Journal
Issue number2
StatePublished - 1995
Externally publishedYes


Dive into the research topics of 'Locally formed dopamine modulates renal Na-P(i) co-transport through DA1 and DA2 receptors'. Together they form a unique fingerprint.

Cite this