Localization of cellular antigens in sodium dodecyl sulfate-polyacrylamide gels

W. Steven Adair; Donald Jurivich; Ursula W. Goodenough

doi:10.1083/jcb.79.1.281

Localization of cellular antigens in sodium dodecyl sulfate-polyacrylamide gels

W. Steven Adair, Donald Jurivich, Ursula W. Goodenough

Research output: Contribution to journal › Article › peer-review

73 Scopus citations

Abstract

A procedure is described for localizing antigen-antibody complexes in sodium dodecyl sulfate (SDS) polyacrylamide gels using 125I-labeled protein A from Staphylococcus aureus. We use the procedure to probe antigenic cross-reactivities between Strongylocentrotus and Chlamydomonas α- and β-tubulins; we also demonstrate how the procedure can detect minor antibody species in an antiserum directed against a cell membrane.

Original language	English
Pages (from-to)	281-285
Number of pages	5
Journal	Journal of Cell Biology
Volume	79
Issue number	1
DOIs	https://doi.org/10.1083/jcb.79.1.281
State	Published - 1 Oct 1978
Externally published	Yes

Keywords

Antigen-antibody complexes
Autoradiography
I-protein A
Membranes
Tubulin

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10.1083/jcb.79.1.281

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abstract = "A procedure is described for localizing antigen-antibody complexes in sodium dodecyl sulfate (SDS) polyacrylamide gels using 125I-labeled protein A from Staphylococcus aureus. We use the procedure to probe antigenic cross-reactivities between Strongylocentrotus and Chlamydomonas α- and β-tubulins; we also demonstrate how the procedure can detect minor antibody species in an antiserum directed against a cell membrane.",

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AB - A procedure is described for localizing antigen-antibody complexes in sodium dodecyl sulfate (SDS) polyacrylamide gels using 125I-labeled protein A from Staphylococcus aureus. We use the procedure to probe antigenic cross-reactivities between Strongylocentrotus and Chlamydomonas α- and β-tubulins; we also demonstrate how the procedure can detect minor antibody species in an antiserum directed against a cell membrane.

KW - Antigen-antibody complexes

KW - Autoradiography

KW - I-protein A

KW - Membranes

KW - Tubulin

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Localization of cellular antigens in sodium dodecyl sulfate-polyacrylamide gels

Abstract

Keywords

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