TY - JOUR
T1 - Local myocardial overexpression of growth hormone attenuates postinfarction remodeling and preserves cardiac function
AU - Jayasankar, Vasant
AU - Bish, Lawrence T.
AU - Pirolli, Timothy J.
AU - Berry, Mark F.
AU - Burdick, Jeffrey
AU - Woo, Y. Joseph
N1 - Funding Information:
This work was supported in part by grant HL69597 from the National Heart, Lung, and Blood Institute, National Institutes of Health (Bethesda, MD).
PY - 2004/6
Y1 - 2004/6
N2 - Background Ventricular remodeling with chamber dilation and wall thinning is seen in postinfarction heart failure. Growth hormone induces myocardial hypertrophy when oversecreted. We hypothesized that localized myocardial hypertrophy induced by gene transfer of growth hormone could inhibit remodeling and preserve cardiac function after myocardial infarction. Methods Rats underwent direct intramyocardial injection of adenovirus encoding either human growth hormone (n = 9) or empty null vector as control (n = 9) 3 weeks after ligation of the left anterior descending coronary artery. Analysis of the following was performed 3 weeks after delivery: hemodynamics, ventricular geometry, cardiomyocyte fiber size, and serum growth hormone levels. Results The growth hormone group had significantly better systolic cardiac function as measured by maximum left ventricular pressure (73.6 ± 6.9 mm Hg versus control 63.7 ± 7.8 mm Hg, p < 0.05) and maximum dP/dt (2845 ± 453 mm Hg/s versus 1949 ± 605 mm Hg/s, p < 0.005), and diastolic function as measured by minimum dP/dt (-2,520 ± 402 mm Hg/s versus -1,500 ± 774 mm Hg/s, p < 0.01). Ventricular geometry was preserved in the growth hormone group (ventricular diameter 12.2 ± 0.7 mm versus control 13.1 ± 0.4 mm, p < 0.05; borderzone wall thickness 2.0 ± 0.2 mm versus 1.5 ± 0.1 mm, p < 0.001), and was associated with cardiomyocyte hypertrophy (6.09 ± 0.63 μm versus 4.66 ± 0.55 μm, p < 0.005). Local myocardial expression of growth hormone was confirmed, whereas serum levels were undetectable after 3 weeks. Conclusions Local myocardial overexpression of growth hormone after myocardial infarction resulted in cardiomyocyte hypertrophy, attenuated ventricular remodeling, and improved systolic and diastolic cardiac function. The induction of localized myocardial hypertrophy presents a novel therapeutic approach for the treatment of ischemic heart failure.
AB - Background Ventricular remodeling with chamber dilation and wall thinning is seen in postinfarction heart failure. Growth hormone induces myocardial hypertrophy when oversecreted. We hypothesized that localized myocardial hypertrophy induced by gene transfer of growth hormone could inhibit remodeling and preserve cardiac function after myocardial infarction. Methods Rats underwent direct intramyocardial injection of adenovirus encoding either human growth hormone (n = 9) or empty null vector as control (n = 9) 3 weeks after ligation of the left anterior descending coronary artery. Analysis of the following was performed 3 weeks after delivery: hemodynamics, ventricular geometry, cardiomyocyte fiber size, and serum growth hormone levels. Results The growth hormone group had significantly better systolic cardiac function as measured by maximum left ventricular pressure (73.6 ± 6.9 mm Hg versus control 63.7 ± 7.8 mm Hg, p < 0.05) and maximum dP/dt (2845 ± 453 mm Hg/s versus 1949 ± 605 mm Hg/s, p < 0.005), and diastolic function as measured by minimum dP/dt (-2,520 ± 402 mm Hg/s versus -1,500 ± 774 mm Hg/s, p < 0.01). Ventricular geometry was preserved in the growth hormone group (ventricular diameter 12.2 ± 0.7 mm versus control 13.1 ± 0.4 mm, p < 0.05; borderzone wall thickness 2.0 ± 0.2 mm versus 1.5 ± 0.1 mm, p < 0.001), and was associated with cardiomyocyte hypertrophy (6.09 ± 0.63 μm versus 4.66 ± 0.55 μm, p < 0.005). Local myocardial expression of growth hormone was confirmed, whereas serum levels were undetectable after 3 weeks. Conclusions Local myocardial overexpression of growth hormone after myocardial infarction resulted in cardiomyocyte hypertrophy, attenuated ventricular remodeling, and improved systolic and diastolic cardiac function. The induction of localized myocardial hypertrophy presents a novel therapeutic approach for the treatment of ischemic heart failure.
KW - 30
UR - http://www.scopus.com/inward/record.url?scp=2542458277&partnerID=8YFLogxK
U2 - 10.1016/j.athoracsur.2003.12.043
DO - 10.1016/j.athoracsur.2003.12.043
M3 - Article
C2 - 15172279
AN - SCOPUS:2542458277
SN - 0003-4975
VL - 77
SP - 2122
EP - 2129
JO - Annals of Thoracic Surgery
JF - Annals of Thoracic Surgery
IS - 6
ER -