TY - JOUR
T1 - Live attenuated influenza virus vaccines strains with temperature-sensitive defects in P3 protein and nucleoprotein
AU - Palese, P.
AU - Ritchey, Mary B.
N1 - Funding Information:
ACKNOWLEDGMENTS The authors wish to thank Drs. Chanock, Murphy, and Spring for generously providing us with HK-ts-l[E] virus, its parent viruses, and the recombinants derived from HK-ts-l[E] virus and A/AA/ Marton/ virus, and for sharing unpublished information from their laboratory. The authors also wish to thank Ms. Marlene Lin for her expert technical assistance. This work was made possible by grants from the National Institutes of Health (AI-11823) and from the National Science Foundation (PCM-76-11066). M. B. R. was supported by a postdoctoral fellowship from the National Institutes of Health (IF32 AI-05229-OIAI).
PY - 1977/5/1
Y1 - 1977/5/1
N2 - The temperature-sensitive influenza virus A/Hong Kong/68 (H3N2) ts-1[E] has been used as a prototype live attenuated influenza virus vaccine. Using recently developed techniques to map the genome of influenza viruses and to "genotype" influenza virus recombinants, the temperature-sensitive lesions in the virus were identified. These defects, responsible for the attenuation of the virus, are located in the genes for the P3 protein and the nucleoprotein and are associated with virus-specific RNA synthesis. Hong Kong/68 (H3N2) ts-1[E] virus can also serve as a donor of "attenuation characteristics" for the selection of recombinant strains which have different surface antigens and may be used as vaccine strains in the future. The temperature-sensitive mutations of Hong Kong/68 (H3N2) ts-1[E] virus were previously transferred to recombinant viruses carrying the HO hemagglutinin. The RNAs of 8 of these temperature-sensitive recombinants were analyzed. One of these viruses, R1, classified in group 1 of the Hong Kong mutant virus set was found to possess a ts defect only in the P3 protein. R8, a member of group 2 of the Hong Kong mutant virus set had a ts mutation in the nucleoprotein.
AB - The temperature-sensitive influenza virus A/Hong Kong/68 (H3N2) ts-1[E] has been used as a prototype live attenuated influenza virus vaccine. Using recently developed techniques to map the genome of influenza viruses and to "genotype" influenza virus recombinants, the temperature-sensitive lesions in the virus were identified. These defects, responsible for the attenuation of the virus, are located in the genes for the P3 protein and the nucleoprotein and are associated with virus-specific RNA synthesis. Hong Kong/68 (H3N2) ts-1[E] virus can also serve as a donor of "attenuation characteristics" for the selection of recombinant strains which have different surface antigens and may be used as vaccine strains in the future. The temperature-sensitive mutations of Hong Kong/68 (H3N2) ts-1[E] virus were previously transferred to recombinant viruses carrying the HO hemagglutinin. The RNAs of 8 of these temperature-sensitive recombinants were analyzed. One of these viruses, R1, classified in group 1 of the Hong Kong mutant virus set was found to possess a ts defect only in the P3 protein. R8, a member of group 2 of the Hong Kong mutant virus set had a ts mutation in the nucleoprotein.
UR - http://www.scopus.com/inward/record.url?scp=0017737567&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(77)90090-3
DO - 10.1016/0042-6822(77)90090-3
M3 - Article
C2 - 860398
AN - SCOPUS:0017737567
SN - 0042-6822
VL - 78
SP - 183
EP - 191
JO - Virology
JF - Virology
IS - 1
ER -