Liquid to liquid extraction and liquid chromatography-tandem mass spectrometry determination of hainanmycin in feed

Ze Ping Wang, Jian Zhong Shen, Robert J. Linhardt, Hui Jiang, Lin Li Cheng

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Hainanmycin is a new veterinary polyether antibiotic and has few sensitive analytical method in present days. In this study, a liquid chromatography-tandem mass spectrometry (LC–MS/MS) relying on multiple reaction monitoring (MRM) detection was developed for analysis of hainanmycin in animal feed. Feed samples were extracted with ethyl acetate and purified by two steps of liquid-liquid extraction (LLE) to get rid of water solvable matrix and lipids one by one. The final simple was analyzed by LC–MS/MS. The LC mobile phase was composed of 0.1% aqueous formic acid and 0.1% formic acidified acetonitrile by gradient elution. Average recoveries ranged from 74.22% to 87.85%, as determined by spiking with 2.0 (LOQ) ∼2500 μg kg−1 of hainanmycin. The inter-day and intra-day coefficient of variation was 9.21% to 11.77% and 7.67% to 13.49%, respectively. The limit of detection (LOD) and the limit of quantitation (LOQ) were 0.36 μg kg−1 and 2.0 μg kg−1, respectively.

Original languageEnglish
Pages (from-to)98-101
Number of pages4
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume1046
DOIs
StatePublished - 1 Mar 2017
Externally publishedYes

Keywords

  • Feed
  • Hainanmycin
  • Liquid chromatography–tandem mass spectrometry
  • Sample preparation

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