Liquid nitrogen cryospray for biological applications

Franck Felisaz; Ricardo Steinmann; Andrew Thompson; Vivian Stojanoff

doi:10.1016/S0921-4526(99)02850-1

Liquid nitrogen cryospray for biological applications

Franck Felisaz
, Ricardo Steinmann
, Andrew Thompson
, Vivian Stojanoff

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

One of the methods to study three-dimensional protein structures is via X-ray diffraction techniques. Radiation damage produced by the interaction of X-rays with the sample can be reduced by soaking the protein crystals in cryoprotectants and freezing them at liquid nitrogen temperature. The perfection of the frozen crystals depends critically on the choice of cryoprotectant, and the rate of freezing. We have developed a device to produce a fine spray of liquid-nitrogen droplets smaller than 10 μm in order to increase the rate of freezing.

Original language	English
Pages (from-to)	2047-2048
Number of pages	2
Journal	Physica B: Condensed Matter
Volume	284-288
Issue number	PART II
DOIs	https://doi.org/10.1016/S0921-4526(99)02850-1
State	Published - 2000
Externally published	Yes

Keywords

Cryogenic instrumentation
Cryoprotection
Nitrogen cryo spray
Protein structure

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10.1016/S0921-4526(99)02850-1

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title = "Liquid nitrogen cryospray for biological applications",

abstract = "One of the methods to study three-dimensional protein structures is via X-ray diffraction techniques. Radiation damage produced by the interaction of X-rays with the sample can be reduced by soaking the protein crystals in cryoprotectants and freezing them at liquid nitrogen temperature. The perfection of the frozen crystals depends critically on the choice of cryoprotectant, and the rate of freezing. We have developed a device to produce a fine spray of liquid-nitrogen droplets smaller than 10 μm in order to increase the rate of freezing.",

keywords = "Cryogenic instrumentation, Cryoprotection, Nitrogen cryo spray, Protein structure",

author = "Franck Felisaz and Ricardo Steinmann and Andrew Thompson and Vivian Stojanoff",

year = "2000",

doi = "10.1016/S0921-4526(99)02850-1",

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volume = "284-288",

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publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Liquid nitrogen cryospray for biological applications

AU - Felisaz, Franck

AU - Steinmann, Ricardo

AU - Thompson, Andrew

AU - Stojanoff, Vivian

PY - 2000

Y1 - 2000

N2 - One of the methods to study three-dimensional protein structures is via X-ray diffraction techniques. Radiation damage produced by the interaction of X-rays with the sample can be reduced by soaking the protein crystals in cryoprotectants and freezing them at liquid nitrogen temperature. The perfection of the frozen crystals depends critically on the choice of cryoprotectant, and the rate of freezing. We have developed a device to produce a fine spray of liquid-nitrogen droplets smaller than 10 μm in order to increase the rate of freezing.

AB - One of the methods to study three-dimensional protein structures is via X-ray diffraction techniques. Radiation damage produced by the interaction of X-rays with the sample can be reduced by soaking the protein crystals in cryoprotectants and freezing them at liquid nitrogen temperature. The perfection of the frozen crystals depends critically on the choice of cryoprotectant, and the rate of freezing. We have developed a device to produce a fine spray of liquid-nitrogen droplets smaller than 10 μm in order to increase the rate of freezing.

KW - Cryogenic instrumentation

KW - Cryoprotection

KW - Nitrogen cryo spray

KW - Protein structure

UR - https://www.scopus.com/pages/publications/0033702121

U2 - 10.1016/S0921-4526(99)02850-1

DO - 10.1016/S0921-4526(99)02850-1

M3 - Article

AN - SCOPUS:0033702121

SN - 0921-4526

VL - 284-288

SP - 2047

EP - 2048

JO - Physica B: Condensed Matter

JF - Physica B: Condensed Matter

IS - PART II

ER -

Liquid nitrogen cryospray for biological applications

Abstract

Keywords

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