Lipoprotein lipase secretion from isolated rat fat cells of different size

Susan K. Fried; Mario DiGirolamo

doi:10.1016/0024-3205(86)90363-2

Lipoprotein lipase secretion from isolated rat fat cells of different size

Susan K. Fried, Mario DiGirolamo

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Lipoprotein lipase(LPL) release from isolated small fat cells from young rats and large fat cells from older, fatter rats was compared during in vitro incubation at 30°C. Although large fat cells had nearly three times higher cellular LPL activity, they secreted similar amounts of LPL activity into the incubation medium under both basal conditions (Krebs Ringer bicarbonate buffer containing 4% albumin and 6mM glucose) and after stimulation of LPL release by 5% human serum, or serum plus 1 U/ml herapin. These data suggest that previous observations of an altered tissue distribution of LPL in adipose tissue containing large fat cells can be at least in part explained by an alteration at the level of LPL secretion.

Original language	English
Pages (from-to)	2111-2119
Number of pages	9
Journal	Life Sciences
Volume	39
Issue number	22
DOIs	https://doi.org/10.1016/0024-3205(86)90363-2
State	Published - 1 Dec 1986
Externally published	Yes

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@article{06ccecdf9ae94b1784f3762e45285451,

title = "Lipoprotein lipase secretion from isolated rat fat cells of different size",

abstract = "Lipoprotein lipase(LPL) release from isolated small fat cells from young rats and large fat cells from older, fatter rats was compared during in vitro incubation at 30°C. Although large fat cells had nearly three times higher cellular LPL activity, they secreted similar amounts of LPL activity into the incubation medium under both basal conditions (Krebs Ringer bicarbonate buffer containing 4% albumin and 6mM glucose) and after stimulation of LPL release by 5% human serum, or serum plus 1 U/ml herapin. These data suggest that previous observations of an altered tissue distribution of LPL in adipose tissue containing large fat cells can be at least in part explained by an alteration at the level of LPL secretion.",

author = "Fried, {Susan K.} and Mario DiGirolamo",

note = "Funding Information: This work was supported Luke's/Roosevelt Institute for 06600 and AM26687.",

year = "1986",

month = dec,

day = "1",

doi = "10.1016/0024-3205(86)90363-2",

language = "English",

volume = "39",

pages = "2111--2119",

journal = "Life Sciences",

issn = "0024-3205",

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number = "22",

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T1 - Lipoprotein lipase secretion from isolated rat fat cells of different size

AU - Fried, Susan K.

AU - DiGirolamo, Mario

N1 - Funding Information: This work was supported Luke's/Roosevelt Institute for 06600 and AM26687.

PY - 1986/12/1

Y1 - 1986/12/1

N2 - Lipoprotein lipase(LPL) release from isolated small fat cells from young rats and large fat cells from older, fatter rats was compared during in vitro incubation at 30°C. Although large fat cells had nearly three times higher cellular LPL activity, they secreted similar amounts of LPL activity into the incubation medium under both basal conditions (Krebs Ringer bicarbonate buffer containing 4% albumin and 6mM glucose) and after stimulation of LPL release by 5% human serum, or serum plus 1 U/ml herapin. These data suggest that previous observations of an altered tissue distribution of LPL in adipose tissue containing large fat cells can be at least in part explained by an alteration at the level of LPL secretion.

AB - Lipoprotein lipase(LPL) release from isolated small fat cells from young rats and large fat cells from older, fatter rats was compared during in vitro incubation at 30°C. Although large fat cells had nearly three times higher cellular LPL activity, they secreted similar amounts of LPL activity into the incubation medium under both basal conditions (Krebs Ringer bicarbonate buffer containing 4% albumin and 6mM glucose) and after stimulation of LPL release by 5% human serum, or serum plus 1 U/ml herapin. These data suggest that previous observations of an altered tissue distribution of LPL in adipose tissue containing large fat cells can be at least in part explained by an alteration at the level of LPL secretion.

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U2 - 10.1016/0024-3205(86)90363-2

DO - 10.1016/0024-3205(86)90363-2

M3 - Article

C2 - 3784772

AN - SCOPUS:0022998372

SN - 0024-3205

VL - 39

SP - 2111

EP - 2119

JO - Life Sciences

JF - Life Sciences

IS - 22

ER -

Lipoprotein lipase secretion from isolated rat fat cells of different size

Abstract

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