Lipopolysaccharide, but not IFN-γ, down-regulates c-fms mRNA proto-oncogene expression in murine macrophages

Gabriele L. Gusella, Emira Ayroldi, Igor Espinoza-Delgado, Luigi Varesio

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28 Scopus citations


In the present study we analyzed the effects of two macrophage activators, bacterial LPS and IFN-γ, on the expression of the c-fms proto-oncogene in the immortalized murine macrophage cell line ANA-1. ANA-1 cells constitutively expressed significant levels of c-fms mRNA. LPS stimulation induced down-regulation of the expression of c-fms mRNA. In contrast, IFN-γ did not change c-fms expression. Combined treatment of ANA-1 with IFN-γ plus LPS resulted in a decrease in c-fms mRNA greater than that induced by LPS alone. Nuclear runoff experiments demonstrated that the down-regulation of c-fms mRNA by LPS or LPS plus IFN-γ was controlled at a transcriptional level. Moreover, experiments in which c-fms mRNA expression was evaluated after the block of RNA or of protein synthesis did not reveal any difference in c-fms mRNA stability in LPS-treated and in untreated cells. These results demonstrate that LPS does not affect the stability of c-fms mRNA, but it decreases the transcription of the gene.

Original languageEnglish
Pages (from-to)3574-3580
Number of pages7
JournalJournal of Immunology
Issue number9
StatePublished - 1 May 1990
Externally publishedYes


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