TY - JOUR
T1 - Lipid and bile acid analysis.
AU - Argmann, Carmen A.
AU - Houten, Sander M.
AU - Champy, Marie France
AU - Auwerx, Johan
PY - 2006/8
Y1 - 2006/8
N2 - Lipids are important body constituents that are vital for cellular, tissue, and whole-body homeostasis. Lipids serve as crucial membrane components, constitute the body's main energy reservoir, and are important signaling molecules. As a consequence of these pleiotropic functions, many common diseases, including atherosclerosis, chronic inflammatory disorders, and obesity, have been associated with altered lipid homeostasis. Lipid abnormalities are hence increasingly analyzed in mouse models. This unit describes commonly used methods to analyze mouse lipid metabolism, with techniques that evaluate lipids both in blood and in tissues. Despite the similarities between men and mice in many aspects of metabolism, important differences also exist in the area of lipid homeostasis. These differences are discussed and should be taken into account when extrapolating lipid data from mouse to men.
AB - Lipids are important body constituents that are vital for cellular, tissue, and whole-body homeostasis. Lipids serve as crucial membrane components, constitute the body's main energy reservoir, and are important signaling molecules. As a consequence of these pleiotropic functions, many common diseases, including atherosclerosis, chronic inflammatory disorders, and obesity, have been associated with altered lipid homeostasis. Lipid abnormalities are hence increasingly analyzed in mouse models. This unit describes commonly used methods to analyze mouse lipid metabolism, with techniques that evaluate lipids both in blood and in tissues. Despite the similarities between men and mice in many aspects of metabolism, important differences also exist in the area of lipid homeostasis. These differences are discussed and should be taken into account when extrapolating lipid data from mouse to men.
UR - http://www.scopus.com/inward/record.url?scp=38649088841&partnerID=8YFLogxK
M3 - Article
C2 - 18265385
AN - SCOPUS:38649088841
SN - 1934-3639
VL - Chapter 29
SP - Unit 29B.2
JO - Current Protocols in Molecular Biology
JF - Current Protocols in Molecular Biology
ER -