TY - JOUR
T1 - Linear density gradient separation of human lymphocyte subsets
AU - Zier, Karen S.
AU - Huber, C. H.
AU - Braunsteiner, H.
N1 - Funding Information:
This work was supported by Austrian “Fonds zur forderung der wissentschaftlichen Forschung.” thank Ms. A. M. Fbdinger for excellent technical help. We are also grateful to Dr. Bernd Gansbacher continuous cooperation.
PY - 1977/6
Y1 - 1977/6
N2 - Linear density gradients were used to separate either unsensitized human peripheral blood lymphocytes or cells sensitized in mixed leukocyte culture (MLC), at different time points in the immune response. Testing of cells from the various density fractions for their ability to respond in MLC and cell‐mediated lympholysis (CML) revealed that (a) activity between individual fractions differed, as well as activity between individual fractions and a suspension of unfractionated cells, (b) although precursor cells of MLC and CML were maximally enriched in closely associated light density regions, their respective distribution profiles were strikingly different, and (c) the density of cells responding in MLC and CML differed depending upon the point of the immune response at which the cells were separated. Whereas effector cells were of light density relative to the precursor cells, putative memory cells for CML exhibited a high density.
AB - Linear density gradients were used to separate either unsensitized human peripheral blood lymphocytes or cells sensitized in mixed leukocyte culture (MLC), at different time points in the immune response. Testing of cells from the various density fractions for their ability to respond in MLC and cell‐mediated lympholysis (CML) revealed that (a) activity between individual fractions differed, as well as activity between individual fractions and a suspension of unfractionated cells, (b) although precursor cells of MLC and CML were maximally enriched in closely associated light density regions, their respective distribution profiles were strikingly different, and (c) the density of cells responding in MLC and CML differed depending upon the point of the immune response at which the cells were separated. Whereas effector cells were of light density relative to the precursor cells, putative memory cells for CML exhibited a high density.
UR - http://www.scopus.com/inward/record.url?scp=0017684497&partnerID=8YFLogxK
U2 - 10.1002/eji.1830070608
DO - 10.1002/eji.1830070608
M3 - Article
C2 - 142641
AN - SCOPUS:0017684497
SN - 0014-2980
VL - 7
SP - 366
EP - 370
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 6
ER -