TY - JOUR
T1 - Linear association between cellular DNA and Epstein Barr virus DNA in a human lymphoblastoid cell line
AU - Adams, A.
AU - Lindahl, T.
AU - Klein, G.
PY - 1973
Y1 - 1973
N2 - High molecular weight DNA from cell line Raji (derived from Burkitt's lymphoma), which contains 50-60 copies of Epstein Barr virus DNA per cell, was fractionated in neutral solution by several cycles of CsCl gradient centrifugation in fixed angle rotors. Under the fractionation conditions used, intact Epstein Barr virus DNA from virus particles can be separated from the less dense cellular DNA. In contrast, a large proportion of the intrinsic Epstein Barr virus DNA component of Raji cells remains associated with cellular DNA, as determined by nucleic acid hybridization. This interaction, which is resistant to Pronase and phenol treatment, is not the result of aggregation. When the molecular weight of Raji DNA is reduced by hydrodynamic shear, the amount of virus DNA associated with cell DNA decreases. However, some virus DNA still remains bound to fragments of cellular DNA after shearing. The association is completely destroyed in alkaline solution. Molecular weight analysis of Raji DNA after denaturation showed that the alkali induced release of Epstein Barr virus DNA was specific and not the result of random single strand breaks. These data indicate that Epstein Barr virus DNA is linearly integrated into Raji cell DNA by alkali labile bonds.
AB - High molecular weight DNA from cell line Raji (derived from Burkitt's lymphoma), which contains 50-60 copies of Epstein Barr virus DNA per cell, was fractionated in neutral solution by several cycles of CsCl gradient centrifugation in fixed angle rotors. Under the fractionation conditions used, intact Epstein Barr virus DNA from virus particles can be separated from the less dense cellular DNA. In contrast, a large proportion of the intrinsic Epstein Barr virus DNA component of Raji cells remains associated with cellular DNA, as determined by nucleic acid hybridization. This interaction, which is resistant to Pronase and phenol treatment, is not the result of aggregation. When the molecular weight of Raji DNA is reduced by hydrodynamic shear, the amount of virus DNA associated with cell DNA decreases. However, some virus DNA still remains bound to fragments of cellular DNA after shearing. The association is completely destroyed in alkaline solution. Molecular weight analysis of Raji DNA after denaturation showed that the alkali induced release of Epstein Barr virus DNA was specific and not the result of random single strand breaks. These data indicate that Epstein Barr virus DNA is linearly integrated into Raji cell DNA by alkali labile bonds.
UR - http://www.scopus.com/inward/record.url?scp=0015894883&partnerID=8YFLogxK
U2 - 10.1073/pnas.70.10.2888
DO - 10.1073/pnas.70.10.2888
M3 - Article
C2 - 4355371
AN - SCOPUS:0015894883
SN - 0027-8424
VL - 70
SP - 2888
EP - 2892
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 10
ER -