@article{b89b70b70e5e4e009eb7b3c1f7b50842,
title = "Limited Regeneration Potential with Minimal Epicardial Progenitor Conversions in the Neonatal Mouse Heart after Injury",
abstract = "The regeneration capacity of neonatal mouse heart is controversial. In addition, whether epicardial cells provide a progenitor pool for de novo heart regeneration is incompletely defined. Following apical resection of the neonatal mouse heart, we observed limited regeneration potential. Fate-mapping of Tbx18MerCreMer mice revealed that newly formed coronary vessels and a limited number of cardiomyocytes were derived from the T-box transcription factor 18 (Tbx18) lineage. However, further lineage tracing with SM-MHCCreERT2 and Nfactc1Cre mice revealed that the new smooth muscle and endothelial cells are in fact derivatives of pre-existing coronary vessels. Our data show that neonatal mouse heart can regenerate but that its potential is limited. Moreover, although epicardial cells are multipotent during embryogenesis, their contribution to heart repair through “stem” or “progenitor” cell conversion is minimal after birth. These observations suggest that early embryonic heart development and postnatal heart regeneration are distinct biological processes. Multipotency of epicardial cells is significantly decreased after birth.",
keywords = "cell lineage tracing, epicardial cells, heart regeneration, neonatal mouse",
author = "Weibin Cai and Jing Tan and Jianyun Yan and Lu Zhang and Xiaoqiang Cai and Haiping Wang and Fang Liu and Maoqing Ye and Cai, {Chen Leng}",
note = "Funding Information: The authors thank Dr. Kevin Kelly in the Transgenic Core of Mount Sinai for generating mouse models. C.-L.C. is supported by grants from NIH (1R01HL131735 and 1R01HL137036), American Heart Association (15GRNT25710153), and NYSTEM (N09G032). W.C. J.Y. and F.L. are supported by grants from the National Natural Science Foundation of China (81670256 and 81741117 to W.C.; 81470488 and 81770280 to J.Y.; and 81670282 to F.L.). C.-L.C. and W.C. designed the study and wrote the paper; W.C. J.T. J.Y. and L.Z. performed the primary experiments and analyzed the data; and X.C. H.W. F.L. and M.Y. provided technical assistance. The authors declare no competing interests. Funding Information: The authors thank Dr. Kevin Kelly in the Transgenic Core of Mount Sinai for generating mouse models. C.-L.C. is supported by grants from NIH ( 1R01HL131735 and 1R01HL137036 ), American Heart Association ( 15GRNT25710153 ), and NYSTEM ( N09G032 ). W.C., J.Y., and F.L. are supported by grants from the National Natural Science Foundation of China ( 81670256 and 81741117 to W.C.; 81470488 and 81770280 to J.Y.; and 81670282 to F.L.). Publisher Copyright: {\textcopyright} 2019 The Author(s)",
year = "2019",
month = jul,
day = "2",
doi = "10.1016/j.celrep.2019.06.003",
language = "English",
volume = "28",
pages = "190--201.e3",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "1",
}