Light and heavy chains specifying a human IgMκ autoantibody to a T-cell receptor Vβ-antigen

Fatma Dedeoglu, Hulya Kaymaz, George Klein, John J. Marchalonis

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Humans frequently produce serum IgM autoantibodies reactive with T-cell receptor β chains at a determinant defined by peptides corresponding to the first complementarity determining region. It is likely that this determinant serves as a public idiotype involved in immunoregulation. Following screening of culture supernatants from over 60 Epstein-Barr virus-carrying B-cell lines of normal and neoplastic origin, we identified a line, IARC307, that secretes an IgMκ protein showing marked specificity for the Vβ8.1 CDR1 sequence CKPISGHNSLFQWYRQT. We cloned and sequenced the complete variable regions of the Vκ and VH chains used by the autoantibody. The light chain has a VκIII sequence related to the 'a' subgroup and uses Jκ2. The heavy chain has a VHIII sequence essentially identical to the germline sequence DP54 and uses the JH6C minigene. The CDR3 is unique, differing from those of other autoantibodies. The antibody is rigorously specific in its specificity for the Vβ8 peptide and does not show polyspecificity for protein or DNA antigens.

Original languageEnglish
Pages (from-to)223-227
Number of pages5
JournalImmunology Letters
Volume38
Issue number3
DOIs
StatePublished - Nov 1993
Externally publishedYes

Keywords

  • Gene sequence
  • Heavy chain variable domain
  • Light chain variable domain
  • Polymerase chain reaction

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