LC-MS determination and pharmacokinetic study of salidroside in rat plasma after oral administration of traditional chinese medicinal preparation rhodiola crenulata extract

Juan Zhang, Xiaohui Chen, Peng Wang, Lin Huo, Zhenduo Shen, Xiaorui Guo, Weiming Cheng, Kaishun Bi

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

A simple, rapid and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed for the quantification of salidroside in rat plasma and the study of its pharmacokinetics after oral administration of 15 g kg -1 Rhodiola crenulata extract to Wistar rats. A 200 μL plasma sample was extracted by acetonitrile and performed on Kromasil C18 column (150 mm × 4.6 mm, 5 μm) with the mobile phase of acetonitrile-water (11:89) within a run time of 8 min. The analyte was monitored with electrospray ionization (ESI) by selected ion monitoring (SIM) mode. The target ions were m/z 299.20 for salidroside and m/z 150.00 for internal standard (IS) paracetamol. A good linear relationship was obtained over the range of 100-20,000 ng mL-1 and the lower limit of quantification was 100 ng mL-1. The validated method was successfully applied for the pharmacokinetic study of salidroside in rat. After oral administration of Rhodiola crenulata extract, the main pharmacokinetic parameters T max, T 1/2, C max, AUC 0-t and AUC 0-∞ were 0.56 ± 0.21 h, 7.91 ± 4.42 h, 3,386 ± 2,138 ng mL-1, 16,146 ± 6,558 ng h mL-1 and 18,599 ± 6,529 ng h mL-1, respectively.

Original languageEnglish
Pages (from-to)695-700
Number of pages6
JournalChromatographia
Volume67
Issue number9-10
DOIs
StatePublished - May 2008
Externally publishedYes

Keywords

  • Column liquid chromatography-mass spectrometry
  • Pharmacokinetic study
  • Rhodiola crenulata
  • Salidroside in rat plasma

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