TY - JOUR
T1 - LC-MS determination and pharmacokinetic study of salidroside in rat plasma after oral administration of traditional chinese medicinal preparation rhodiola crenulata extract
AU - Zhang, Juan
AU - Chen, Xiaohui
AU - Wang, Peng
AU - Huo, Lin
AU - Shen, Zhenduo
AU - Guo, Xiaorui
AU - Cheng, Weiming
AU - Bi, Kaishun
PY - 2008/5
Y1 - 2008/5
N2 - A simple, rapid and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed for the quantification of salidroside in rat plasma and the study of its pharmacokinetics after oral administration of 15 g kg -1 Rhodiola crenulata extract to Wistar rats. A 200 μL plasma sample was extracted by acetonitrile and performed on Kromasil C18 column (150 mm × 4.6 mm, 5 μm) with the mobile phase of acetonitrile-water (11:89) within a run time of 8 min. The analyte was monitored with electrospray ionization (ESI) by selected ion monitoring (SIM) mode. The target ions were m/z 299.20 for salidroside and m/z 150.00 for internal standard (IS) paracetamol. A good linear relationship was obtained over the range of 100-20,000 ng mL-1 and the lower limit of quantification was 100 ng mL-1. The validated method was successfully applied for the pharmacokinetic study of salidroside in rat. After oral administration of Rhodiola crenulata extract, the main pharmacokinetic parameters T max, T 1/2, C max, AUC 0-t and AUC 0-∞ were 0.56 ± 0.21 h, 7.91 ± 4.42 h, 3,386 ± 2,138 ng mL-1, 16,146 ± 6,558 ng h mL-1 and 18,599 ± 6,529 ng h mL-1, respectively.
AB - A simple, rapid and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed for the quantification of salidroside in rat plasma and the study of its pharmacokinetics after oral administration of 15 g kg -1 Rhodiola crenulata extract to Wistar rats. A 200 μL plasma sample was extracted by acetonitrile and performed on Kromasil C18 column (150 mm × 4.6 mm, 5 μm) with the mobile phase of acetonitrile-water (11:89) within a run time of 8 min. The analyte was monitored with electrospray ionization (ESI) by selected ion monitoring (SIM) mode. The target ions were m/z 299.20 for salidroside and m/z 150.00 for internal standard (IS) paracetamol. A good linear relationship was obtained over the range of 100-20,000 ng mL-1 and the lower limit of quantification was 100 ng mL-1. The validated method was successfully applied for the pharmacokinetic study of salidroside in rat. After oral administration of Rhodiola crenulata extract, the main pharmacokinetic parameters T max, T 1/2, C max, AUC 0-t and AUC 0-∞ were 0.56 ± 0.21 h, 7.91 ± 4.42 h, 3,386 ± 2,138 ng mL-1, 16,146 ± 6,558 ng h mL-1 and 18,599 ± 6,529 ng h mL-1, respectively.
KW - Column liquid chromatography-mass spectrometry
KW - Pharmacokinetic study
KW - Rhodiola crenulata
KW - Salidroside in rat plasma
UR - http://www.scopus.com/inward/record.url?scp=43549096634&partnerID=8YFLogxK
U2 - 10.1365/s10337-008-0585-7
DO - 10.1365/s10337-008-0585-7
M3 - Article
AN - SCOPUS:43549096634
SN - 0009-5893
VL - 67
SP - 695
EP - 700
JO - Chromatographia
JF - Chromatographia
IS - 9-10
ER -