LC method for the determination of multiple components in a compound cold formulation

An isocratic liquid chromatographic method for determination of acetaminophen (AMP), caffeine (CAF), chlorphenamine maleate (CPM) and guaiacol glyceryl ether (GGE) in a compound cold formulation is described. Separation and quantitation were achieved on a Diamonsil C18 column using a binary mixture of methanol and 1.5% aqueous acetic acid (55: 45, v/v, pH 3.6) as mobile phase delivered at 0.4 ml. min^-1. Single wavelength detection was at 220 nm for all four drugs and the run time was < 10 min. The linearity, accuracy and precision of the method were found to be acceptable over the concentration ranges: 16.0-127.8 μg mL^-1 for AMP, 6.0-48.2 μg mL^-1 for CAF, 5.0-40.0 μg mL^-1 for CPM and 10.1-80.6 μg mL ^-1 for GGE.