Late measures of brain injury after neonatal hypoxia-ischemia in mice

Vadim S. Ten, Ed X. Wu, Haiying Tang, Maria Bradley-Moore, Maksim V. Fedarau, Veniamin I. Ratner, Raymond I. Stark, Jay A. Gingrich, David J. Pinsky

Research output: Contribution to journalArticlepeer-review

85 Scopus citations


Background and Purpose-This work was undertaken to determine to what degree long-term neurofunctional outcome of neonatal hypoxic-ischemic (HI) brain injury in mice correlates with anatomical extent of cerebral damage assessed by magnetic resonance imaging (MRI) and histopathology. Methods-On postnatal day 7, mice were subjected to HI. At 7 to 9 weeks after HI neurofunctional outcome was assessed by water-maze, rota-rod, and open-field test performance, followed by cerebral MRI and histopathology evaluation. Results-At 10 weeks after HI, MRI revealed ipsilateral brain atrophy alone or with porencephalic cyst formation and contralateral ventriculomegaly. Adult HI-affected mice, especially those that developed a porencephalic cyst, demonstrated significant neurofunctional deficit compared with age-matched naïve mice. HI-affected mice with ipsilateral cerebral atrophy but without porencephaly demonstrated no or an intermediate level of neurofunctional deficit. Neurobehavioral assessment of mice subjected to HI insult revealed a strong correlation between degree of brain injury and functional neurohandicap. Conclusions-This is the first study to demonstrate that long-term neurofunctional outcome in mice after a neonatal HI correlates tightly with anatomical pattern/extent of cerebral damage, defined by MRI and histopathology.

Original languageEnglish
Pages (from-to)2183-2188
Number of pages6
Issue number9
StatePublished - Sep 2004
Externally publishedYes


  • Animals, newborn
  • Hypoxia
  • Ischemia
  • Mice


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