Lactate release from isolated rat adipocytes: Influence of cell size, glucose concentration, insulin and epinephrine

D. L. Crandall, S. K. Fried, A. A. Francendese, M. Nickel, M. DiGirolamo

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Release of lactate was studied during in vitro incubations with isolated fat cells. Lactate release increased (approximately 3-fold) with increasing medium glucose concentration (from 3 to 12 mM) in both large and small fat cells. Large fat cells from older, fatter rats, however, released 3-4 times more lactate per cell than small fat cells from young rats when incubated with 3, 6, or 12 mM glucose. Insulin and epinephrine produced a marked stimulation of lactate release in small fat cells, but these hormones had no effect in large fat cells. Lactate accounted for only 10-15% of the glucose metabolized by small fat cells under all incubation conditions but was nearly 40% of glucose utilized by large fat cells at glucose concentrations greater than 6 mM. In conclusion, lactate is a major metabolite of glucose in adipocytes, particularly in the large fat cells. Adipose tissue may therefore be a major site of lactate production, particularly in states of altered glucose metabolism (i.e., hyperglycemia) and obesity.

Original languageEnglish
Pages (from-to)326-329
Number of pages4
JournalUnknown Journal
Volume15
Issue number7
DOIs
StatePublished - 1983
Externally publishedYes

Fingerprint

Dive into the research topics of 'Lactate release from isolated rat adipocytes: Influence of cell size, glucose concentration, insulin and epinephrine'. Together they form a unique fingerprint.

Cite this