Labeling of sarcoma associated monoclonal antibody with 111In, 67Ga and 125I

E. Lavie, M. Boazi, J. Weininger, M. Bitton, G. Yosilevski, D. Front, Y. Hirshaut, E. Robinson, A. H. Bartal

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4 Scopus citations

Abstract

Labeling of human sarcoma-associated murine monoclonal antibody (MAb) 23H7 with 67Ga and 111In by the bifunctional ligand method is reported. 67Ga was chelated to the MAb via desferrioxamine B and 111In via the cyclic anhydride of DTPA. Higher specific activity was obtained with 67Ga (4-5 μCi/μg) as compared with 111In (2 μCi/μg). The binding capacity of the MAb was confirmed by repeated indirect immuno-fluorescence assays performed before and after labeling. A fast blood clearance was observed: 33% recovered dose (R.D.) blood level 3 h post-injection as compared with 56% after injection of control polyclonal IgG. Preliminary results on chemically induced sarcoma bearing mice showed a relatively high tumor uptake of the labeled antibody.

Original languageEnglish
Pages (from-to)129-135
Number of pages7
JournalRadiotherapy and Oncology
Volume8
Issue number2
DOIs
StatePublished - 1987
Externally publishedYes

Keywords

  • Monoclonal antibody labeling
  • Sarcoma

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