LAB: A new membrane-associated adaptor molecule in B cell activation

Erin Janssen; Minghua Zhu; Weijia Zhang; Surapong Koonpaew; Weiguo Zhang

doi:10.1038/ni882

LAB: A new membrane-associated adaptor molecule in B cell activation

Erin Janssen, Minghua Zhu, Weijia Zhang, Surapong Koonpaew, Weiguo Zhang

Research output: Contribution to journal › Article › peer-review

139 Scopus citations

Abstract

The adaptor molecule, linker for activation of T cells (LAT), is essential in T cell activation and development; a similar molecule in B cells has not yet been identified. Here, we report the identification of a new adaptor protein, linker for activation of B cells (LAB). Like LAT, LAB was localized to lipid rafts. Upon activation via the B cell receptor (BCR), LAB was phosphorylated and interacted with the adaptor protein Grb2. Decreased LAB expression led to a reduction in BCR-mediated calcium flux and Erk activation. LAB rescued thymocyte development but not normal T cell activation in LAT^-/- mice. Our data suggest that LAB links BCR engagement to downstream signaling pathways.

Original language	English
Pages (from-to)	117-123
Number of pages	7
Journal	Nature Immunology
Volume	4
Issue number	2
DOIs	https://doi.org/10.1038/ni882
State	Published - 1 Feb 2003
Externally published	Yes

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title = "LAB: A new membrane-associated adaptor molecule in B cell activation",

abstract = "The adaptor molecule, linker for activation of T cells (LAT), is essential in T cell activation and development; a similar molecule in B cells has not yet been identified. Here, we report the identification of a new adaptor protein, linker for activation of B cells (LAB). Like LAT, LAB was localized to lipid rafts. Upon activation via the B cell receptor (BCR), LAB was phosphorylated and interacted with the adaptor protein Grb2. Decreased LAB expression led to a reduction in BCR-mediated calcium flux and Erk activation. LAB rescued thymocyte development but not normal T cell activation in LAT-/- mice. Our data suggest that LAB links BCR engagement to downstream signaling pathways.",

author = "Erin Janssen and Minghua Zhu and Weijia Zhang and Surapong Koonpaew and Weiguo Zhang",

note = "Funding Information: Acknowledgments We thank the Duke University Cancer Center Flow Cytometry and Sequencing facilities. This work is supported by National Institutes of Health Grant 1R01 AI48674-01.",

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doi = "10.1038/ni882",

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T2 - A new membrane-associated adaptor molecule in B cell activation

AU - Janssen, Erin

AU - Zhu, Minghua

AU - Zhang, Weijia

AU - Koonpaew, Surapong

AU - Zhang, Weiguo

N1 - Funding Information: Acknowledgments We thank the Duke University Cancer Center Flow Cytometry and Sequencing facilities. This work is supported by National Institutes of Health Grant 1R01 AI48674-01.

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N2 - The adaptor molecule, linker for activation of T cells (LAT), is essential in T cell activation and development; a similar molecule in B cells has not yet been identified. Here, we report the identification of a new adaptor protein, linker for activation of B cells (LAB). Like LAT, LAB was localized to lipid rafts. Upon activation via the B cell receptor (BCR), LAB was phosphorylated and interacted with the adaptor protein Grb2. Decreased LAB expression led to a reduction in BCR-mediated calcium flux and Erk activation. LAB rescued thymocyte development but not normal T cell activation in LAT-/- mice. Our data suggest that LAB links BCR engagement to downstream signaling pathways.

AB - The adaptor molecule, linker for activation of T cells (LAT), is essential in T cell activation and development; a similar molecule in B cells has not yet been identified. Here, we report the identification of a new adaptor protein, linker for activation of B cells (LAB). Like LAT, LAB was localized to lipid rafts. Upon activation via the B cell receptor (BCR), LAB was phosphorylated and interacted with the adaptor protein Grb2. Decreased LAB expression led to a reduction in BCR-mediated calcium flux and Erk activation. LAB rescued thymocyte development but not normal T cell activation in LAT-/- mice. Our data suggest that LAB links BCR engagement to downstream signaling pathways.

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LAB: A new membrane-associated adaptor molecule in B cell activation

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