TY - JOUR
T1 - La Piedad Michoacán Mexico Virus V protein antagonizes type I interferon response by binding STAT2 protein and preventing STATs nuclear translocation
AU - Pisanelli, Giuseppe
AU - Laurent-Rolle, Maudry
AU - Manicassamy, Balaji
AU - Belicha-Villanueva, Alan
AU - Morrison, Juliet
AU - Lozano-Dubernard, Bernardo
AU - Castro-Peralta, Felipa
AU - Iovane, Giuseppe
AU - García-Sastre, Adolfo
N1 - Publisher Copyright:
© 2015 Elsevier B.V..
PY - 2016/2/2
Y1 - 2016/2/2
N2 - La Piedad Michoacán Mexico Virus (LPMV) is a member of the Rubulavirus genus within the Paramyxoviridae family. LPMV is the etiologic agent of "blue eye disease", causing a significant disease burden in swine in Mexico with long-term implications for the agricultural industry. This virus mainly affects piglets and is characterized by meningoencephalitis and respiratory distress. It also affects adult pigs, causing reduced fertility and abortions in females, and orchitis and epididymitis in males.Viruses of the Paramyxoviridae family evade the innate immune response by targeting components of the interferon (IFN) signaling pathway. The V protein, expressed by most paramyxoviruses, is a well-characterized IFN signaling antagonist. Until now, there were no reports on the role of the LPMV-V protein in inhibiting the IFN response. In this study we demonstrate that LPMV-V protein antagonizes type I but not type II IFN signaling by binding STAT2, a component of the type I IFN cascade. Our results indicate that the last 18 amino acids of LPMV-V protein are required for binding to STAT2 in human and swine cells. While LPMV-V protein does not affect the protein levels of STAT1 or STAT2, it does prevent the IFN-induced phosphorylation and nuclear translocation of STAT1 and STAT2 thereby inhibiting cellular responses to IFN α/β.
AB - La Piedad Michoacán Mexico Virus (LPMV) is a member of the Rubulavirus genus within the Paramyxoviridae family. LPMV is the etiologic agent of "blue eye disease", causing a significant disease burden in swine in Mexico with long-term implications for the agricultural industry. This virus mainly affects piglets and is characterized by meningoencephalitis and respiratory distress. It also affects adult pigs, causing reduced fertility and abortions in females, and orchitis and epididymitis in males.Viruses of the Paramyxoviridae family evade the innate immune response by targeting components of the interferon (IFN) signaling pathway. The V protein, expressed by most paramyxoviruses, is a well-characterized IFN signaling antagonist. Until now, there were no reports on the role of the LPMV-V protein in inhibiting the IFN response. In this study we demonstrate that LPMV-V protein antagonizes type I but not type II IFN signaling by binding STAT2, a component of the type I IFN cascade. Our results indicate that the last 18 amino acids of LPMV-V protein are required for binding to STAT2 in human and swine cells. While LPMV-V protein does not affect the protein levels of STAT1 or STAT2, it does prevent the IFN-induced phosphorylation and nuclear translocation of STAT1 and STAT2 thereby inhibiting cellular responses to IFN α/β.
KW - (LPMV) La Piedad Michoacan Mexico Virus
KW - Interferon-signaling antagonist
KW - LPMV-V
KW - STAT2
UR - http://www.scopus.com/inward/record.url?scp=84958758698&partnerID=8YFLogxK
U2 - 10.1016/j.virusres.2015.10.027
DO - 10.1016/j.virusres.2015.10.027
M3 - Article
C2 - 26546155
AN - SCOPUS:84958758698
SN - 0168-1702
VL - 213
SP - 11
EP - 22
JO - Virus Research
JF - Virus Research
ER -