l-Baclofen-sensitive GABAB binding sites in the medial vestibular nucleus localized by immunocytochemistry

Gay R. Holstein; Giorgio P. Martinelli; Bernard Cohen

doi:10.1016/0006-8993(92)90361-C

l-Baclofen-sensitive GABA_B binding sites in the medial vestibular nucleus localized by immunocytochemistry

Gay R. Holstein
, Giorgio P. Martinelli
, Bernard Cohen

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

l-Baclofen-sensitive GABA_B binding sites in the medial vestibular nucleus (MVN) were identified immunocytochemically and visualized ultrastructurally in l-baclofen-preinjected rats and monkeys, using a mouse monoclonal antibody with specificity for the p-chlorophenyl moiety of baclofen. Saline-preinjected animals showed no immunostain. In drug-injected animals, there was evidence for both pre- and postsynaptic GABAergic inhibition in MVN mediated by GABA_B receptors. These neural elements could be utilized in control of velocity storage in the vestibulo-ocular reflex.

Original language	English
Pages (from-to)	175-180
Number of pages	6
Journal	Brain Research
Volume	581
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(92)90361-C
State	Published - 22 May 1992

Keywords

Baclofen
GABA receptor
Medial vestibular nucleus
Vestibulo-ocular reflex
γ-Aminobutyric acid

Access to Document

10.1016/0006-8993(92)90361-C

Cite this

@article{78fbd955c31b4d909eabee1c4860f047,

title = "l-Baclofen-sensitive GABAB binding sites in the medial vestibular nucleus localized by immunocytochemistry",

abstract = "l-Baclofen-sensitive GABAB binding sites in the medial vestibular nucleus (MVN) were identified immunocytochemically and visualized ultrastructurally in l-baclofen-preinjected rats and monkeys, using a mouse monoclonal antibody with specificity for the p-chlorophenyl moiety of baclofen. Saline-preinjected animals showed no immunostain. In drug-injected animals, there was evidence for both pre- and postsynaptic GABAergic inhibition in MVN mediated by GABAB receptors. These neural elements could be utilized in control of velocity storage in the vestibulo-ocular reflex.",

keywords = "Baclofen, GABA receptor, Medial vestibular nucleus, Vestibulo-ocular reflex, γ-Aminobutyric acid",

author = "Holstein, \{Gay R.\} and Martinelli, \{Giorgio P.\} and Bernard Cohen",

note = "Funding Information: Aided by NIH Grants NS-24656 and NS-00294. We thank Ciba-Geigy, Ltd. for providing the L-baclofen. We thank Dr. Harel Weinstein for helpful discussion relating to this research.",

year = "1992",

month = may,

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doi = "10.1016/0006-8993(92)90361-C",

language = "English",

volume = "581",

pages = "175--180",

journal = "Brain Research",

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T1 - l-Baclofen-sensitive GABAB binding sites in the medial vestibular nucleus localized by immunocytochemistry

AU - Holstein, Gay R.

AU - Martinelli, Giorgio P.

AU - Cohen, Bernard

N1 - Funding Information: Aided by NIH Grants NS-24656 and NS-00294. We thank Ciba-Geigy, Ltd. for providing the L-baclofen. We thank Dr. Harel Weinstein for helpful discussion relating to this research.

PY - 1992/5/22

Y1 - 1992/5/22

N2 - l-Baclofen-sensitive GABAB binding sites in the medial vestibular nucleus (MVN) were identified immunocytochemically and visualized ultrastructurally in l-baclofen-preinjected rats and monkeys, using a mouse monoclonal antibody with specificity for the p-chlorophenyl moiety of baclofen. Saline-preinjected animals showed no immunostain. In drug-injected animals, there was evidence for both pre- and postsynaptic GABAergic inhibition in MVN mediated by GABAB receptors. These neural elements could be utilized in control of velocity storage in the vestibulo-ocular reflex.

AB - l-Baclofen-sensitive GABAB binding sites in the medial vestibular nucleus (MVN) were identified immunocytochemically and visualized ultrastructurally in l-baclofen-preinjected rats and monkeys, using a mouse monoclonal antibody with specificity for the p-chlorophenyl moiety of baclofen. Saline-preinjected animals showed no immunostain. In drug-injected animals, there was evidence for both pre- and postsynaptic GABAergic inhibition in MVN mediated by GABAB receptors. These neural elements could be utilized in control of velocity storage in the vestibulo-ocular reflex.

KW - Baclofen

KW - GABA receptor

KW - Medial vestibular nucleus

KW - Vestibulo-ocular reflex

KW - γ-Aminobutyric acid

UR - https://www.scopus.com/pages/publications/0026762488

U2 - 10.1016/0006-8993(92)90361-C

DO - 10.1016/0006-8993(92)90361-C

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