Ku70: A candidate tumor suppressor gene for murine T cell lymphoma

Gloria C. Li; Honghai Ouyang; Xiaoling Li; Hatsumi Nagasawa; John B. Little; David J. Chen; C. Clifton Ling; Zvi Fuks; Carlos Cordon-Cardo

doi:10.1016/s1097-2765(00)80108-2

Ku70: A candidate tumor suppressor gene for murine T cell lymphoma

Gloria C. Li
, Honghai Ouyang
, Xiaoling Li
, Hatsumi Nagasawa
, John B. Little
, David J. Chen
, C. Clifton Ling
, Zvi Fuks
, Carlos Cordon-Cardo

Research output: Contribution to journal › Article › peer-review

196 Scopus citations

Abstract

We present evidence that inactivation of the Ku70 gene leads to a propensity for malignant transformation both in vitro and in vivo. In vitro, Ku70^-/- mouse fibroblasts displayed an increased rate of sister chromatid exchange and a high frequency of spontaneous neoplastic transformation. In vivo, Ku70^-/- mice, known to be defective in B but not T lymphocyte maturation, developed thymic and disseminated T cell lymphomas at a mean age of 6 months with CD4⁺CD8⁺ tumor cells. These findings directly demonstrate that Ku70 deficiency facilitates neoplastic growth and suggest a novel role of the Ku70 locus in tumor suppression.

Original language	English
Pages (from-to)	1-8
Number of pages	8
Journal	Molecular Cell
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/s1097-2765(00)80108-2
State	Published - Jul 1998
Externally published	Yes

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@article{f54c142bb68d480390e27c22be70eca5,

title = "Ku70: A candidate tumor suppressor gene for murine T cell lymphoma",

abstract = "We present evidence that inactivation of the Ku70 gene leads to a propensity for malignant transformation both in vitro and in vivo. In vitro, Ku70-/- mouse fibroblasts displayed an increased rate of sister chromatid exchange and a high frequency of spontaneous neoplastic transformation. In vivo, Ku70-/- mice, known to be defective in B but not T lymphocyte maturation, developed thymic and disseminated T cell lymphomas at a mean age of 6 months with CD4+CD8+ tumor cells. These findings directly demonstrate that Ku70 deficiency facilitates neoplastic growth and suggest a novel role of the Ku70 locus in tumor suppression.",

author = "Li, \{Gloria C.\} and Honghai Ouyang and Xiaoling Li and Hatsumi Nagasawa and Little, \{John B.\} and Chen, \{David J.\} and Ling, \{C. Clifton\} and Zvi Fuks and Carlos Cordon-Cardo",

note = "Funding Information: We thank A. Kurimasa for PCR primers, J.S. Danska for TCR Cβ cDNA probe, D. Kim for affinity-purified Ku70 antiserum, M.S. Jiao, Nai-Kong Cheung, Minglan Lu, Xiyun Shao, Min Guo, Jianlin Xu, and Sa Wang for technical assistance, T. Deloherey for FACS analysis, P. Krechmer for word processing, and R. Chaganti for assistance in karyotyping of the Ku70 −/− lymphoma cells and for valuable suggestions. The work was supported in part by National Institutes of Health grants CA-31397 and CA-56909 (G. C. L.) and the Office of Biological and Environmental Research/Department of Energy (D. C.).",

year = "1998",

month = jul,

doi = "10.1016/s1097-2765(00)80108-2",

language = "English",

volume = "2",

pages = "1--8",

journal = "Molecular Cell",

issn = "1097-2765",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - Ku70

T2 - A candidate tumor suppressor gene for murine T cell lymphoma

AU - Li, Gloria C.

AU - Ouyang, Honghai

AU - Li, Xiaoling

AU - Nagasawa, Hatsumi

AU - Little, John B.

AU - Chen, David J.

AU - Ling, C. Clifton

AU - Fuks, Zvi

AU - Cordon-Cardo, Carlos

N1 - Funding Information: We thank A. Kurimasa for PCR primers, J.S. Danska for TCR Cβ cDNA probe, D. Kim for affinity-purified Ku70 antiserum, M.S. Jiao, Nai-Kong Cheung, Minglan Lu, Xiyun Shao, Min Guo, Jianlin Xu, and Sa Wang for technical assistance, T. Deloherey for FACS analysis, P. Krechmer for word processing, and R. Chaganti for assistance in karyotyping of the Ku70 −/− lymphoma cells and for valuable suggestions. The work was supported in part by National Institutes of Health grants CA-31397 and CA-56909 (G. C. L.) and the Office of Biological and Environmental Research/Department of Energy (D. C.).

PY - 1998/7

Y1 - 1998/7

N2 - We present evidence that inactivation of the Ku70 gene leads to a propensity for malignant transformation both in vitro and in vivo. In vitro, Ku70-/- mouse fibroblasts displayed an increased rate of sister chromatid exchange and a high frequency of spontaneous neoplastic transformation. In vivo, Ku70-/- mice, known to be defective in B but not T lymphocyte maturation, developed thymic and disseminated T cell lymphomas at a mean age of 6 months with CD4+CD8+ tumor cells. These findings directly demonstrate that Ku70 deficiency facilitates neoplastic growth and suggest a novel role of the Ku70 locus in tumor suppression.

AB - We present evidence that inactivation of the Ku70 gene leads to a propensity for malignant transformation both in vitro and in vivo. In vitro, Ku70-/- mouse fibroblasts displayed an increased rate of sister chromatid exchange and a high frequency of spontaneous neoplastic transformation. In vivo, Ku70-/- mice, known to be defective in B but not T lymphocyte maturation, developed thymic and disseminated T cell lymphomas at a mean age of 6 months with CD4+CD8+ tumor cells. These findings directly demonstrate that Ku70 deficiency facilitates neoplastic growth and suggest a novel role of the Ku70 locus in tumor suppression.

UR - https://www.scopus.com/pages/publications/0032113987

U2 - 10.1016/s1097-2765(00)80108-2

DO - 10.1016/s1097-2765(00)80108-2

M3 - Article

C2 - 9702186

AN - SCOPUS:0032113987

SN - 1097-2765

VL - 2

SP - 1

EP - 8

JO - Molecular Cell

JF - Molecular Cell

IS - 1

ER -

Ku70: A candidate tumor suppressor gene for murine T cell lymphoma

Abstract

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