Kinetic Response of Human Lymphoid Cells to Adriamycin-DNA Complex in Vitro

Barthel Barlogie; Benjamin Drewinko; Robert S. Benjamin; Emil J. Freireich

Kinetic Response of Human Lymphoid Cells to Adriamycin-DNA Complex in Vitro

Barthel Barlogie, Benjamin Drewinko, Robert S. Benjamin, Emil J. Freireich

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The lysosomotropic agent adriamycin-DNA complex was analyzed for its effect on cell cycle progression of human lymphoid cells in culture by means of pulse cytophotometry. Complexing to DNA slightly reduced the perturbation effects previously reported for adriamycin alone. The major kinetic response was a G₂block, the magnitude and duration of which was dependent on drug concentration and duration of treatment. When high drug concentrations were maintained for a prolonged period of time, an additional, completely reversible block in G, phase or at the G,-S boundary was observed, accounting for two-step G₂accumulation curves. Cell age markedly influenced the magnitude of G₂accumulation in that treatment of cells in S and early G₂phase was most effective.

Original language	English
Pages (from-to)	2450-2454
Number of pages	5
Journal	Cancer Research
Volume	36
State	Published - Jul 1976
Externally published	Yes

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title = "Kinetic Response of Human Lymphoid Cells to Adriamycin-DNA Complex in Vitro",

abstract = "The lysosomotropic agent adriamycin-DNA complex was analyzed for its effect on cell cycle progression of human lymphoid cells in culture by means of pulse cytophotometry. Complexing to DNA slightly reduced the perturbation effects previously reported for adriamycin alone. The major kinetic response was a G2block, the magnitude and duration of which was dependent on drug concentration and duration of treatment. When high drug concentrations were maintained for a prolonged period of time, an additional, completely reversible block in G, phase or at the G,-S boundary was observed, accounting for two-step G2accumulation curves. Cell age markedly influenced the magnitude of G2accumulation in that treatment of cells in S and early G2phase was most effective.",

author = "Barthel Barlogie and Benjamin Drewinko and Benjamin, {Robert S.} and Freireich, {Emil J.}",

year = "1976",

month = jul,

language = "English",

volume = "36",

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journal = "Cancer Research",

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T1 - Kinetic Response of Human Lymphoid Cells to Adriamycin-DNA Complex in Vitro

AU - Barlogie, Barthel

AU - Drewinko, Benjamin

AU - Benjamin, Robert S.

AU - Freireich, Emil J.

PY - 1976/7

Y1 - 1976/7

N2 - The lysosomotropic agent adriamycin-DNA complex was analyzed for its effect on cell cycle progression of human lymphoid cells in culture by means of pulse cytophotometry. Complexing to DNA slightly reduced the perturbation effects previously reported for adriamycin alone. The major kinetic response was a G2block, the magnitude and duration of which was dependent on drug concentration and duration of treatment. When high drug concentrations were maintained for a prolonged period of time, an additional, completely reversible block in G, phase or at the G,-S boundary was observed, accounting for two-step G2accumulation curves. Cell age markedly influenced the magnitude of G2accumulation in that treatment of cells in S and early G2phase was most effective.

AB - The lysosomotropic agent adriamycin-DNA complex was analyzed for its effect on cell cycle progression of human lymphoid cells in culture by means of pulse cytophotometry. Complexing to DNA slightly reduced the perturbation effects previously reported for adriamycin alone. The major kinetic response was a G2block, the magnitude and duration of which was dependent on drug concentration and duration of treatment. When high drug concentrations were maintained for a prolonged period of time, an additional, completely reversible block in G, phase or at the G,-S boundary was observed, accounting for two-step G2accumulation curves. Cell age markedly influenced the magnitude of G2accumulation in that treatment of cells in S and early G2phase was most effective.

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M3 - Article

AN - SCOPUS:0017118869

SN - 0008-5472

VL - 36

SP - 2450

EP - 2454

JO - Cancer Research

JF - Cancer Research

ER -

Kinetic Response of Human Lymphoid Cells to Adriamycin-DNA Complex in Vitro

Abstract

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