Isolation of UV-sensitive clones from a haploid frog cell line

Barry Rosenstein, Betsy M. Ohlsson-Wilhelm

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18 Scopus citations


An isolation procedure has been developed which yielded five clones of haploid frog cells which are sensitive to ultraviolet light. This procedure employed a conventional mutagenesis, followed by time for phenotypic expression and then an enrichment for UV-sensitive mutants. The enrichment relies upon the uptake of bromodeoxyuridine (BrdU) by repairing cells following UV-induced damage, rendering repair-proficient cells differentially sensitive to photolysis by black light. The photolysis is potentiated by use of the bisbenzimidazole dye Hoechst 33258. The enriched population was screened for radiation-sensitive isolates resulting in 5 sensitives from 96 tested. No mutants were obtained from 300 isolates tested from a population which had not undergone enrichment.

Original languageEnglish
Pages (from-to)117-128
Number of pages12
JournalSomatic Cell Genetics
Issue number1
StatePublished - Jan 1979
Externally publishedYes


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