TY - JOUR
T1 - Isolation of two Chinese bovine enteroviruses and sequence analysis of their complete genomes
AU - Li, Yingli
AU - Chang, Jitao
AU - Wang, Qian
AU - Yu, Li
N1 - Funding Information:
This work was supported by grants from the National Key Laboratory of Veterinary Biotechnology (SKLVBP201029) and Special Fund for Agro-scientific Research in the Public Interest of China (No. 200803018).
PY - 2012/12
Y1 - 2012/12
N2 - In this study, RNA corresponding to bovine enterovirus (BEV) was detected in 24. 6 % of faecal samples (17/69) from diarrheic and healthy cattle in six different areas in China by an RT-PCR screening method. Furthermore, two cytopathic agents, designated as BHM26 and BJ50, were isolated from the bovine diarrheic fecal samples. During passage in MA104 cells, ultrathin sections of virus-infected monolayers were examined using a transmission electron microscope, and a large number of symmetrical virus crystals were seen in the cytoplasm, with monomorphic small viral particles of 27-30 nm in diameter. The full-length RNA genomes were 7433 and 7416 nucleotides long, respectively, with a genome organization analogous to that of picornaviruses. Phylogenetic analysis of the VP1 and VP3 capsid protein coding sequences suggested that the viruses BHM26 and BJ50 belong to genotype 2 of the BEV cluster B (BEV-B). In addition, sequence comparisons of the 5′ and 3′ UTRs and P1, P2 and P3 subgenomic regions of the two isolates suggested that there were intergenotypic recombination events occurring during evolution of the BHM26 and BJ50 isolates.
AB - In this study, RNA corresponding to bovine enterovirus (BEV) was detected in 24. 6 % of faecal samples (17/69) from diarrheic and healthy cattle in six different areas in China by an RT-PCR screening method. Furthermore, two cytopathic agents, designated as BHM26 and BJ50, were isolated from the bovine diarrheic fecal samples. During passage in MA104 cells, ultrathin sections of virus-infected monolayers were examined using a transmission electron microscope, and a large number of symmetrical virus crystals were seen in the cytoplasm, with monomorphic small viral particles of 27-30 nm in diameter. The full-length RNA genomes were 7433 and 7416 nucleotides long, respectively, with a genome organization analogous to that of picornaviruses. Phylogenetic analysis of the VP1 and VP3 capsid protein coding sequences suggested that the viruses BHM26 and BJ50 belong to genotype 2 of the BEV cluster B (BEV-B). In addition, sequence comparisons of the 5′ and 3′ UTRs and P1, P2 and P3 subgenomic regions of the two isolates suggested that there were intergenotypic recombination events occurring during evolution of the BHM26 and BJ50 isolates.
UR - http://www.scopus.com/inward/record.url?scp=84870291590&partnerID=8YFLogxK
U2 - 10.1007/s00705-012-1424-6
DO - 10.1007/s00705-012-1424-6
M3 - Article
C2 - 22851010
AN - SCOPUS:84870291590
SN - 0304-8608
VL - 157
SP - 2369
EP - 2375
JO - Archives of Virology
JF - Archives of Virology
IS - 12
ER -