Isolation of temperature-sensitive mutants of murine leukemia virus

John R. Stephenson; Roberta K. Reynolds; Stuart A. Aaronson

doi:10.1016/0042-6822(72)90158-4

Isolation of temperature-sensitive mutants of murine leukemia virus

John R. Stephenson
, Roberta K. Reynolds
, Stuart A. Aaronson

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

A microtiter technique was developed which allowed screening of large numbers of clones of murine leukemia virus for the presence of temperature-sensitive mutants. Potential mutants were detected by their inability to induce XC syncytium formation at the nonpermissive temperature. A total of 9 mutant viruses which have a markedly reduced infectivity at the nonpermissive temperature have been isolated. The spontaneous rate of reversion of each mutant was found to be very low.

Original language	English
Pages (from-to)	749-756
Number of pages	8
Journal	Virology
Volume	48
Issue number	3
DOIs	https://doi.org/10.1016/0042-6822(72)90158-4
State	Published - Jun 1972
Externally published	Yes

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title = "Isolation of temperature-sensitive mutants of murine leukemia virus",

abstract = "A microtiter technique was developed which allowed screening of large numbers of clones of murine leukemia virus for the presence of temperature-sensitive mutants. Potential mutants were detected by their inability to induce XC syncytium formation at the nonpermissive temperature. A total of 9 mutant viruses which have a markedly reduced infectivity at the nonpermissive temperature have been isolated. The spontaneous rate of reversion of each mutant was found to be very low.",

author = "Stephenson, \{John R.\} and Reynolds, \{Roberta K.\} and Aaronson, \{Stuart A.\}",

year = "1972",

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journal = "Virology",

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T1 - Isolation of temperature-sensitive mutants of murine leukemia virus

AU - Stephenson, John R.

AU - Reynolds, Roberta K.

AU - Aaronson, Stuart A.

PY - 1972/6

Y1 - 1972/6

N2 - A microtiter technique was developed which allowed screening of large numbers of clones of murine leukemia virus for the presence of temperature-sensitive mutants. Potential mutants were detected by their inability to induce XC syncytium formation at the nonpermissive temperature. A total of 9 mutant viruses which have a markedly reduced infectivity at the nonpermissive temperature have been isolated. The spontaneous rate of reversion of each mutant was found to be very low.

AB - A microtiter technique was developed which allowed screening of large numbers of clones of murine leukemia virus for the presence of temperature-sensitive mutants. Potential mutants were detected by their inability to induce XC syncytium formation at the nonpermissive temperature. A total of 9 mutant viruses which have a markedly reduced infectivity at the nonpermissive temperature have been isolated. The spontaneous rate of reversion of each mutant was found to be very low.

UR - https://www.scopus.com/pages/publications/0015348585

U2 - 10.1016/0042-6822(72)90158-4

DO - 10.1016/0042-6822(72)90158-4

M3 - Article

C2 - 4337991

AN - SCOPUS:0015348585

SN - 0042-6822

VL - 48

SP - 749

EP - 756

JO - Virology

JF - Virology

IS - 3

ER -

Isolation of temperature-sensitive mutants of murine leukemia virus

Abstract

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