Abstract
Automated single-cell dispensing is incompatible with white adipose tissue (WAT) due to lipid-laden adipocytes. Single-nuclei RNA-Seq permits transcriptional profiling of all cells from WAT. Human WAT faces unique technical challenges in isolating nuclei compared to rodent tissue due to greater extra-cellular matrix content and larger lipid droplets. In this protocol, we detail how to isolate nuclei from frozen subcutaneous human WAT for single-nuclei RNA-Seq. For complete information on the generation and use of this protocol, please refer to Whytock et al. (2022).1
Original language | English |
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Article number | 102054 |
Journal | STAR Protocols |
Volume | 4 |
Issue number | 1 |
DOIs | |
State | Published - 17 Mar 2023 |
Keywords
- Cell isolation
- Metabolism
- RNAseq
- Single Cell