Isolation of mouse primary aortic endothelial cells by selection with specific antibodies

Pedro Molina-Sánchez, Vicente Andrés

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

6 Scopus citations

Abstract

Endothelial cells (ECs) are key blood-vessel-wall components that play critical roles in the regulation of many physiological processes, including angiogenesis, coagulation, and vascular tone control, and in pathological events such as vessel inammation and leukocyte infiltration. EC dysfunction is one of the first events associated with the development of atherosclerosis and is sustained throughout progression of the disease. The study of ECs in vitro has become an invaluable tool for investigating these vascular processes at the molecular level, and is widely used in the search for therapeutic targets and strategies.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages111-117
Number of pages7
DOIs
StatePublished - 2015

Publication series

NameMethods in Molecular Biology
Volume1339
ISSN (Print)1064-3745

Keywords

  • Antibody-mediated selection
  • Atherosclerosis
  • Culture
  • Endothelial cells
  • Isolation
  • Mouse aorta

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